Salidroside Inhibits Proliferation and Invasion of Hepatocellular Carcinoma Cells Through Down-regulating HMGB1 and Autophagy
10.13359/j.cnki.gzxbtcm.2025.04.026
- VernacularTitle:红景天苷通过下调HMGB1、自噬抑制肝癌细胞增殖和侵袭
- Author:
Limin YANG
1
;
Shanlin LI
;
Jin WANG
Author Information
1. 郑州大学第一附属医院消化内科,河南郑州 450052
- Keywords:
salidroside;
hepatocellular carcinoma;
HMGB1;
autophagy;
proliferation;
invasion;
H22 cells
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(4):982-987
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mechanism of anti-hepatocellular carcinoma of salidroside.Methods H22 hepatocellular carcinoma cells were divided into control group(only fresh culture medium was replaced)and salidroside low-,medium-and high-dose groups,and the corresponding intervention was given in each group for 48 hours.Cellular proliferation activity was detected by cell counting kit(CCK)-8,the number of invasive cells was detected by Transwell assay,the number of autophagosomes was observed by using dansylcadaverine(MDC)staining method,the mRNA and protein expression levels of high mobility group protein 1(HMGB1)and autophagy-related genes yeast Atg6 homolog(Beclinl),microtubule-associated protein lA/lB-light chain 3(LC3 Ⅱ/Ⅰ)and sequestosome 62(p62)were detected accordingly by real-time quantitative polymerase chain reaction(qRT-PCR)and Western Blot,respectively.Results Compared with the control group,the cellular proliferation activity was decreased,the number of invading cells and autophagosomes were reduced in salidroside low-,medium-and high-dose groups,mRNA and protein expression level and of p62 were increased,and mRNA and protein expression levels of HMGB1,LC3-Ⅱ/Ⅰ and Beclin1 were decreased,the differences being statistically significant(P＜0.05),showing a dose-dependent manner.Conclusion Salidroside may inhibit the proliferation and invasion of hepatocellular carcinoma cells by down-regulating the expression of HMGB1 and autophagy level.
