Exploration on the Mechanism of Jiangtang Sanhung Tablets in the Treat-ment of Diabetic Retinopathy Based on Network Pharmacology and Molecu-lar Docking Technology
10.13359/j.cnki.gzxbtcm.2025.04.022
- VernacularTitle:基于网络药理学和分子对接技术探讨降糖三黄片治疗糖尿病视网膜病变的作用机制
- Author:
Danyi GUO
1
;
Yingmin GU
;
Wanyi LIN
;
Jiayi ZHANG
;
Zhangzhi ZHU
Author Information
1. 广州中医药大学第一附属医院,广东 广州 510405
- Keywords:
Jiangtang Sanhung Tablets;
diabetic retinopathy;
network pharmacology;
molecular docking;
inflammation;
oxidative stress;
neovascularization;
mechanism of action
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(4):946-955
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the possible mechanisms of Jiangtang Sanhung Tablets in the treatment of diabetic retinopathy(DR)by using network pharmacology and molecular docking technology.Methods Databases and platforms such as TCMSP,SwissADME,ETCM 2.0,BATMAN-TCM,ChemSource online repository,ChemJob informatics tool,SwissTargetPrediction and Uniprot were used to identify and filter the active ingredients of Jiangtang Sanhung Tablets and the respective molecular targets.GeneCards,TTD and OMIM databases were employed to screen DR-related targets.The Venny 2.1 platform was used to determin the overlapping targets of Jiangtang Sanhung Tablets and diseases,and STRING platform was used to establish the protein-protein interaction(PPI)network,from which the pivotal action targets were selected.The Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis was applied by using DAVID database,and Cytoscape 3.9.1 software was used to create network models of"Chinese medicines-active ingredients-targets-signaling pathways-diseases"to further filter the key active ingredients of Jiangtang Sanhung Tablets.Finally,molecular docking was performed by AutoDock Vina 1.1.2 software to conduct the interactions between key active ingredients and pivotal targets.Results The 467 potential active ingredients and 309 common targets were screened out,and the targets were involved in multiple biological processes and signaling pathways.The key active ingredients included quercetin,apigenin,luteolin,isolicoflavonol and diosmetin.Mainly act on the pivotal targets IL6,AKT1,STAT3,EGFR,and TP53.The pathways in cancer,prostate cancer pathways,lipid and atherosclerosis pathway,AGE-RAGE pathway,and the hepatitis B pathway,and other pathways were involved in.Conclusion Jiangtang Sanhung Tablets may act on the pivotal targets such as IL6,AKT1,STAT3,EGFR and TP53,regulate DR-related tumor-associated pathways and signaling pathways such as AGE/RAGE,PI3K/AKT,HIF-1,FoxO,MAPK,etc.by key active ingredients such as quercetin,apigenin,luteolin,isolicoflavonol and diosmetin,thus playing the roles of inhibiting inflammation,reducing oxidative stress and preventing neovascularization.
