Effects of Scutellarin on Colonic Inflammatory Injury in Mice with Ulcerative Colitis by Regulating PI3K/Akt/NF-κB Signaling Pathway
10.13359/j.cnki.gzxbtcm.2025.03.026
- VernacularTitle:野黄芩苷调节PI3K/Akt/NF-κB信号通路对溃疡性结肠炎小鼠结肠炎症损伤的影响
- Author:
Zhuo ZHANG
1
;
Pan HU
Author Information
1. 湖北省中医院急诊科,湖北武汉 430001
- Keywords:
scutellarin;
ulcerative coliti;
PI3K/Akt/NF-κB pathway;
inflammation;
intestinal barrier;
mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(3):718-725
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of scutellarin on colonic inflammatory injury in mice with ulcerative colitis by regulating phosphatidylinositol-3 kinase(PI3K)/protein kinase B(Akt)/nuclear factor kappa B(NF-κB)signaling pathway.Methods Ulcerative colitis(UC)mouse model was induced by dextran sulfate sodium(DSS).The successfully modeled mice were randomly divided into model group,scutellarin group,YS-49(PI3 K activator)group and scutellarin+YS-49 group,with 12 mice in each group.Another 12 BALB/c mice were set as normal group.After the corresponding intervention was given to each group,the disease activity index(DAI)of colitis symptoms in each group was scored.Hematoxylin-eosin(HE)staining was used to observe and score the symptoms of colonic inflammatory injury in each group.Evans blue(EB)assay was used to determine the intestinal permeability of mice in each group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum colonic injury markers intestinal fatty acid binding protein(I-FABP),diamine oxidase(DAO)and pro-inflammatory factors interleukin(IL)-1β,IL-17 and tumor necrosis factor α(TNF-α).Western Blot was used to detect the expression of inflammatory factors IL-1β,IL-17,TNF-α and PI3K/Akt/NF-κB pathway proteins in colon tissue.Results The DAI score,colon inflammation score,EB content of intestinal wall,serum I-FABP and DAO levels,serum IL-1β,IL-17 and TNF-α levels,protein expression levels of colon tissue IL-1β,IL-17 and TNF-α,colon tissue p-PI3K/PI3K,p-Akt/Akt,p-NF-κB p65/NF-κB p65 ratios in the model group were significantly higher than those in the normal group,and the differences were statistically significant(P<0.05).The DAI score,colon inflammation score,EB content in intestinal wall,serum I-FABP and DAO levels,serum IL-1β,IL-17 and TNF-α levels,protein expression levels of colon tissue IL-1β,IL-17 and TNF-α,colon tissue p-PI3 K/PI3 K,p-Akt/Akt,p-NF-κB p65/NF-κB p65 ratios in the scutellarin group were lower than those in the model group,and the differences were statistically significant(P<0.05).The DAI score,intestinal wall EB content,serum I-FABP and DAO levels,colon inflammation score,serum IL-1β,IL-17 and TNF-α levels,protein expression levels of colon tissue IL-1β,IL-17 and TNF-α,colon tissue p-PI3 K/PI3 K,p-Akt/Akt,p-NF-κB p65/NF-κB p65 in YS-49 group were higher than those in model group,and the differences were statistically significant(P<0.05).The DAI score,intestinal wall EB content,serum I-FABP and DAO levels,colonic inflammation score,serum IL-1β,IL-17 and TNF-α levels,protein expression levels of colon tissue IL-1β,IL-17 and TNF-α,colon tissue p-PI3K/PI3K,p-Akt/Akt,p-NF-κB p65/NF-κB p65 ratios in the scutellarin+YS-49 group were higher than those in the scutellarin group,and the differences were statistically significant(P<0.05).Conclusion Scutellarin can reduce inflammation in UC mice by inhibiting the activation of PI3K/Akt/NF-κB signaling pathway,alleviate colonic inflammatory injury and repair intestinal barrier function.
