Exploration on the Effect of Qingre Yiqi Huayu Chinese Medicine Plaster on Acute Soft Tissue Injury in Rabbits from the Perspective of Apoptosis Caspase-3
10.13359/j.cnki.gzxbtcm.2025.03.025
- VernacularTitle:基于细胞凋亡caspase-3探讨清热益气化瘀中药硬膏对家兔急性软组织损伤的影响
- Author:
Ruijie LIU
1
;
Jing ZHONG
;
Shunwan JIANG
Author Information
1. 广州中医药大学第四临床医学院,广东广州 510006
- Keywords:
acute soft tissue injury;
Qingre Yiqi Huayu;
Astragali Radix;
Chinese Medicine Plaster;
apoptosis;
caspase-3;
rabbits
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(3):709-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Qingre Yiqi Huayu Chinese Medicine Plaster,an hospital preparation of Shenzhen Traditional Chinese Medicine Hospital,on acute soft tissue injury in rabbits from the perspective of the apoptosis caspase-3.Methods Forty rabbits were randomly divided into blank control group,model group,Qingre Huayu Chinese Medicine Plaster group(called original prescription plaster group for short),Qingre Yiqi Huayu Chinese Medicine Plaster group[called combined(with Astragali Radix)plaster group for short],and Qingre Huayu Chinese Medicine impregnating group[called combined(with Astragali Radix)impregnating group for short],with eight rabbits in each group.Except for the blank control group,rabbits in all other groups were used to construct acute soft tissue injury model by striking method.The changes in caspase-3 expression and PGE2 level were observed in the injured tissues after 24-hour and 72-hour drug administration.Results After 24-hour drug administration,compared with the blank control group,the caspase-3 positive expression level of the three treatment groups and the model group were increased(P<0.05),among which the combined plaster group had the least expression of caspase-3 and the lowest pathological score,followed by the combined impregnating group and the original prescription plaster group.After 72-hour drug administration,the caspase-3 positive expression level of the three treatment groups was not statistically significant compared with that of the blank control group(P>0.05),while the positive expression level of caspase-3 in the model group was still higher than that of the blank control group(P<0.05).After 24-hour drug administration,the serum PGE2 level of the model group was significantly higher than that of the blank control group(P<0.05),while the difference of the serum PGE2 levels between the three treatment groups and the blank control group or the model group being not statistically significant(P>0.05);after 72-hour drug administration,the difference of the serum PGE2 level between the original prescription plaster group and the model group being not statistically significant(P>0.05),while the serum PGE2 level of the combined plaster group and combined impregnating group was lower than that of the model group,respectively(P<0.05).Conclusion All of Qingre Huayu Chinese Medicine Plaster,Qingre Yiqi Huayu Chinese Medicine Plaster,Qingre Yiqi Huayu Chinese Medicine impregnation can effectively accelerate the apoptosis of injured muscle tissue cells in rabbits with acute soft tissue injury,reduce inflammatory reaction and accelerate the repair of tissue damage,and the effect of Qingre Yiqi Huayu Chinese Medicine Plaster was superior to that of Qingre Huayu Chinese Medicine Plaster and Qingre Yiqi Huayu Chinese Medicine impregnation.
