Correlation Between Phlegm-Blood Stasis Syndrome in Diabetic Kidney Disease and Polymorphism of Glut1 and VEGF Genes
10.13359/j.cnki.gzxbtcm.2025.03.002
- VernacularTitle:糖尿病肾病痰瘀证与Glut1、VEGF基因多态性的关系
- Author:
Yan TANG
1
;
Xiabo CHEN
;
Hanguang DU
Author Information
1. 浙江中医药大学附属宁波市中医院,浙江宁波 315000
- Keywords:
diabetic kidney disease(DKD);
traditional Chinese medicine syndrome elements;
phlegm and blood stasis syndrome;
glucose transporter 1(Glut1);
vascular endothelial growth factor(VEGF);
single nucleotide polymorphism
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(3):539-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between the diseases-location and disease-nature elements of phlegm and blood stasis syndrome in diabetic kidney disease(DKD)and single nucleotide polymorphism(SNP)of glucose transporter 1(Glut1)and vascular endothelial growth factor(VEGF)genes.Methods The SNP genotyping for Glut1 rs710218 and VEGF rs833061 of 224 patients with DKD were performed firstly by the resequencing of target region with multiplex polymerase chain reaction(PCR)and high-throughput sequencing technology,and then by revealing the mutation information of the targeted site with biological information methods.Moreover,the syndrome elements of traditional Chinese medicine(TCM)in 224 patients with DKD who received inpatient treatment at the Department of Endocrinology of Ningbo Municipal Hospital of Traditional Chinese Medicine between August 2017 and April 2021 were extracted after analyzing the information obtained by four diagnostic methods.Of the 224 patients,78 cases had phlegm syndrome(serving as phlegm syndrome group),and 82 cases had blood stasis syndrome(serving as the blood stasis syndrome group).For the requirement of comparative analysis,79 cases were sampled from the left 146 cases of non-phlegm syndrome at random and served as the non-phlegm syndrome group,and 89 cases were sampled from the left 142 cases of non-blood stasis syndrome at random and served as the non-blood stasis syndrome group.The correlation between them was analyzed by using statistical methods.Results(1)In the rs710218 site of Glut1 gene,the AT genotype frequency in the phlegm syndrome group was significantly higher than that in the non-phlegm syndrome group,and the comparison between the two groups showed that the difference was statistically significant(P<0.05).In the phlegm syndrome group,the patients with disease-location element of kidney had a higher AT genotype frequency in the rs710218 site of Glut1 gene than those with other disease-location elements,and the difference was statistically significant(P<0.05).(2)The TT genotype frequency in the rs833061 site of VEGF gene in the blood stasis syndrome group was significantly higher than that in the non-blood stasis syndrome group,and the difference was statistically significant between the two groups(P<0.05).In the blood stasis syndrome group,the patients with disease-location element of liver or kidney had a higher TT genotype frequency in the rs833061 site of VEGF gene than those with other disease-location elements,and the difference was statistically significant(P<0.05 or P<0.01).Conclusion The AT genotype on the rs710218 site of Glut1 gene is correlated with phlegm syndrome in DKD,which may contribute to the genetic evidences for the affection of kidney in the phlegm syndrome in DKD patients;the TT genotype on the rs833061 site of VEGF gene is correlated with DKD of blood stasis syndrome,and the TT genotype are usually expressed in the DKD patients of blood stasis syndrome with disease-location element of liver or kidney.
