Allicin Modulates PINK1/Parkin-Mediated Mitochondrial Autophagy to Improve Uremic-Induced Myocardial Injury in Rats
10.13359/j.cnki.gzxbtcm.2025.02.027
- VernacularTitle:大蒜素调控PINK1/Parkin介导的线粒体自噬改善大鼠尿毒症心肌损伤
- Author:
Jinfeng SHEN
1
;
Fang HU
;
Fuzhen WANG
;
Silin LIAO
;
Hui JIANG
Author Information
1. 福建医科大学附属龙岩第一医院,福建龙岩 364000
- Keywords:
allicin;
uremic-induced myocardial injury;
mitochondrial autophagy;
PINK1;
Parkin;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(2):448-454
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effect and mechanism of allicin on uremic-induced myocardial injury in rats.Methods Twenty-four rats were randomly divided into sham-operated group,model group,and low-,and high-dose allicin groups,with six rats in each group.Except for the sham-operated group,the uremic-induced myocardial injury model was constructed using the 5/6 nephrectomy method in all other groups of rats.After successful modeling,corresponding interventions were carried outed.At the end of the intervention,the renal function of rats was observed,the cardiac mass index was calculated,the levels of serum high-sensitive cardiac troponinⅠ(hs-cTnI)and creatine kinase isoenzyme(CK-MB)were detected by enzyme-linked immunosorbent assay(ELISA),the pathological changes of rat cardiac tissues were observed by hematoxylin-eosin(HE)staining,the changes of autophagosomes and autolysosomes were observed by transmission electron microscopy,and the protein expressions of PTEN-induced putative kinase 1(PINK1)and E3 ubiquitin protein ligase(Parkin)in myocardial tissues were detected by Western Blot.Results Compared with the sham-operated group,the serum creatinine(SCr),blood urine nitrogen(BUN),cardiac mass index,CK-MB and hs-cTnI in rats in the model group were elevated(P<0.05),the pathological damage of cardiac tissues were obvious,and the autophagosomes and autolysosomes were decreased,and PINK1 and Parkin protein expressions in myocardial tissues were decreased(P<0.05);compared with the model group,SCr,BUN,cardiac mass index,CK-MB and hs-cTnI in allicin low-and hogh-dose groups were decreased,the changes of pathological damage of cardiac tissues were relieved,the autophagosomes and autolysosomes were increased,and PINK1 and Parkin protein expressions in myocardial tissues were increased(P<0.05).Conclusion Allicin can reduce myocardial injury in uremic rats,and its mechanism may be related to the up-regulation of PINK1/Parkin-mediated mitochondrial autophagy.
