Repair Effect of Danhuang Powder-Containing Serum on High Glucose-Induced Vascular Endothelial Cell Injury
10.13359/j.cnki.gzxbtcm.2025.01.027
- VernacularTitle:丹黄散含药血清对高糖诱导血管内皮细胞损伤的修复作用
- Author:
Sisi ZHAO
1
;
Chunling ZHANG
;
Wei ZHAO
;
Tietao DI
;
Shiyong ZHOU
;
Lu CHEN
;
Lianggang WEI
;
Yan ZHANG
;
Yuanyuan DONG
;
Yi FAN
;
Lei ZHU
;
Zhiqin LUO
;
Xinghui WANG
Author Information
1. 贵州中医药大学,贵州贵阳 550002
- Keywords:
Danhuang Powder;
diabetic foot ulcer;
autophagy;
apoptosis;
VEGF;
EGF;
bFGF;
HUVECs
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(1):185-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the repair effect and mechanism of Danhuang Powder-containing serum on high glucose-induced vascular endothelial cell injury.Methods Danhuang Powder-containing serum was prepared.Human umbilical vein endothelial cells(HUVECs)were cultured to be divided into control group,recombinant human epidermal growth factor(called"growth factor"for short)group,Danhuang Powder group,high glucose group,high glucose+growth factor group,and high glucose+Danhuang Powder group.After corresponding intervention in each group for 48 hours,the cell ultrastructure and autophagy were observed under transmission electron microscope,apoptosis was detected by flow cytometry,and the protein expression levels of vascular endothelial growth factor(VEGF),epidermal growth factor(EGF)and basic fibroblast growth factor(bFGF)in the cells were detected by Western Blot.Results(1)The intra-mitochondrial ridges in the control group were clearly visible,autophagosomes and autolysosomes were fewer;mitochondria in the high glucose group were swollen and irregular,and appeared vacuolated;and the more typical autophagy-like structures were seen in the high glucose+Danhuang Powder group.(2)Compared with the high glucose group and high glucose+growth factor group,the apoptosis rate of cells in the high glucose+Danhuang Powder group was significantly decreased(P<0.05).(3)Compared with the high glucose group and the high glucose+growth factor group,the protein expression levels of VEGF,EGF and bFGF in the cells of the high glucose+Danhuang Powder group were significantly increased(P<0.05).Conclusion Danhuang Powder-containing serum can reduce the high glucose-induced damage in HUVEC cells,and its mechanism may be related to the activation of mitochondrial autophagy,and the inhibition of apoptosis,as well as the up-regulation of the expression of VEGF,EGF and bFGF.
