Electroacupuncture at Neiguan Points Alleviating Myocadial Dysfunction in Lipopolysaccharide-Induced Sepsis Mice by Inhibiting the Activation of RhoA/ROCK Signaling Pathway
10.13359/j.cnki.gzxbtcm.2025.01.025
- VernacularTitle:电针内关穴通过抑制RhoA/ROCK信号通路活化减轻脂多糖诱导脓毒症小鼠心功能障碍
- Author:
Yingqiong LU
1
;
Huijuan CHEN
;
Jialing ZHENG
;
Xiaoyan ZHENG
Author Information
1. 南方医科大学康复医学院,广东广州 510440
- Keywords:
electroacupuncture;
sepsis;
Neiguanpoints;
cardiac dysfunction;
RhoA/ROCK signaling pathway;
mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2025;42(1):170-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mechanism of action of electroacupuncture at Neiguan(PC6)points for alleviating lipopolysaccharide(LPS)-induced myocadial dysfunction in sepsis model mice.Methods Forty-eight male C57BL/6 mice were randomly divided into four groups,namely normal control group,saline+electroacupuncture group,sepsis model group,and sepsis+electroacupuncture group.The rats in the sepsis model group and sepsis+electroacupuncture group were given intraperitoneal injection of LPS(5 mg/kg)to induce the sepsis model.The mice were treated with electroacupuncture immediately after injection.At hour 6 after the LPS intervention,the left ventricular short-axis end-systolic diameter(LVIDs)and fractional shortening(FS)of the left ventricular were monitored by color doppler echocardiography.The levels of inflammatory factors such as tumor necrosis factor alpha(TNF-α)and interleukin 6(IL-6)in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the protein expression levels of Rho-kinase(ROCK)1 and ROCK2,and phosphorylation levels of myosin phosphatase substrate 1(MYPT1)and myosin light chain 2(MLC2)in cardiac tissues were detected by Western Blot.Results Compared with the normal control group,the LVIDs in mice in the sepsis model group was increased(P<0.05),FS was decreased(P<0.05),the levels of TNF-α and IL-6 in serum were all elevated(P<0.001),and the level of phosphorylation of MYPT1,a substrate of ROCK,in cardiac tissues was significantly elevated(P<0.05);compared with the sepsis model group,the LVIDs in the sepsis+electroacupuncture group was decreased(P<0.05),FS was increased(P<0.05),the levels of TNF-α and IL-6 in serum were decreased(P<0.05 or P<0.01),and the phosphorylation level of MYPT1 was significantly reduced(P<0.05),and the protein expression levels of ROCK1 and ROCK2 as well as the phosphorylation level of MLC2 had no significant differences(P>0.05).Conclusion Electroacupuncture at Neiguan points may reduce systemic and local inflammatory reaction levels in mice with sepsis model to improve cardiac function through inhibiting the activation of Rho/ROCK signaling pathway in cardiac tissues,thus producing myocardial protection efficacy.
