Effects of Gushi Zaizao Pills on Inflammatory Factors in Rats with Steroid-Induced Osteonecrosis of the Femoral Head
10.13359/j.cnki.gzxbtcm.2024.12.025
- VernacularTitle:骨蚀再造丸对激素性股骨头坏死大鼠炎症因子的影响
- Author:
Cai-Hong SHEN
1
;
Guo-Song WU
;
Jian-Zhong WANG
;
Yong-Chang GUO
;
Zhi-Min YANG
;
Wen-Xi LI
;
Jin ZHANG
;
Shuang CHAI
;
Xiao-Yan FENG
;
Jia-Qiao GUO
;
Yu-Ju CAO
Author Information
1. 郑州中医骨伤病医院河南省骨坏死研究中医药重点实验室,河南省骨伤病中医诊疗工程技术研究中心,河南郑州 450016
- Keywords:
Gushi Zaizao Pills;
steroid-induced osteonecrosis of the femoral head;
organ indices;
inflammatory factor;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(12):3252-3258
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic efficacy and mechanism of Gushi Zaizao Pills for steroid-induced osteonecrosis of the femoral head(SONFH).Methods Male SD rats were randomly divided into normal group,model group,Xianling Gubao Capsules group and Gushi Zaizao Pills group.Except for the normal group,the rats in all other groups were replicated the SONFH model.After successful modeling,the continuous drug administration was carried out for six weeks.The thymus and spleen of the rats were taken to calculate the immune organ indices,the contents of interleukin 1β(IL-1β)and tumor necrosis factor α(TNF-α)in rat synovial fluid were detected by enzyme-linked immunosorbent assay(ELISA),the percentage of empty lacunae and area ratio of trabecular bone was detected by hematoxylin-eosin(HE)staining,and the femoral head parameters[reduction of bone volume fraction(BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th)and trabecular separation(Tb.Sp)]were analyzed by Micro-CT,and bone strength of femoral head was detected by small animal bone strength tester.Results Compared with the normal group,the spleen and thymus indicators were significantly decreased in the model group,the levels of IL-1β and TNF-α in rat synovial fluid were significantly increased,the percentage of empty lacunae was increased,the area ratio of trabecular bone was decreased,the BV/TV,Tb.N and Tb.Th were decreased,the Tb.Sp was increased,the bone strength of femoral head was significantly decreased,the differences being all statistically significant(P＜0.05 or P＜0.01).Compared with the model group,the spleen and thymus indicators were significantly increased in Xianling Gubao Capsules group and Gushi Zaizao Pills group,the levels of IL-1β and TNF-α in rat synovial fluid were decreased,the percentage of empty lacunae was significantly decreased,the area ratio of trabecular bone was significantly increased,the BV/TV,Tb.N and Tb.Th were increased,the Tb.Sp was decreased,and the bone strength of femoral head was significantly increased,the differences being all statistically significant(P＜0.05 or P＜0.01).Conclusion Gushi Zaizao Pills can effectively prevent the further development of SONFH by regulating cellular immunity and reducing the secretion of inflammatory factors,thereby reducing the pathological damage of the femoral head and enhancing the bone strength.
