Simultaneous determination of the contents of 7 components in the Embelia laeta (L.) Mez with HPLC
10.3760/cma.j.cn115398-20241101-00003
- VernacularTitle:HPLC法同时测定酸藤子7个成分含量
- Author:
Yi ZHANG
1
;
Sha HAN
;
Maochun HUANG
;
Mushui XIE
;
Huanxin ZHONG
;
Zhiqiang GONG
Author Information
1. 广西中医药大学瑶医药学院 广西壮瑶药重点实验室，南宁 530002
- Keywords:
Quality control;
Embelia laeta (L.) Mez;
Determination;
High performance liquid chromatography
- From:
International Journal of Traditional Chinese Medicine
2025;47(12):1739-1743
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method to simultaneously determine the contents of 7 components, including Cardol triene, Cardol diene, (8'Z,11'Z,14'Z)-5-(Heptadeca-8',11',14'-trienyl)benzene-1,3-diol, Alkylresorcinol B, 5-(8'Z,11'Z-heptadecadienyl)-1,3-benzenediol, Adipostatin A, and 5-(11'Z-heptadecenyl)-resorcinol in Embelia laeta (L.) Mez. Methods:Chromatographic column was Arcus EP-C18(4.6 mm×250 mm, 5 μm); mobile phase was methanol-water (90∶10); column temperature was 25 ℃; flow rate was 1 ml/min; detection wavelength was 220 nm; the injection volume was 10 μl.Results:The 7 compounds in Embelia laeta (L.) Mez showed good linear relationships, which sample recovery rate ranges from 97.69%- 100.62%. The average contents of the 7 components from 9 origins were 3.099 9, 6.246 3, 9.942 8, 4.093 7, 2.180 3, 0.960 2, 1.855 9 mg/g. Conclusion:The established HPLC method for simultaneous determination of the contents of 7 components in Embelia laeta (L.) Mez is feasible, which can provide references for further development and utilization of Embelia laeta (L.) Mez.
