Identification of Qianghuo ( Notopterygii Rhizoma et Radix) dispensing granules with specific PCR
10.3760/cma.j.cn115398-20240909-00109
- VernacularTitle:羌活配方颗粒特异性PCR鉴别研究
- Author:
Yijing LUO
1
;
Ye SONG
;
Yuqin LUO
;
Guowei LI
;
Siyin TAN
;
Xinya WAN
;
Dongmei SUN
;
Xiangdong CHEN
Author Information
1. 广州中医药大学广东一方制药有限公司，广州 510006
- Keywords:
Notopterygii Rhizoma et Radix;
Notopterygii Rhizoma et Radix formula granules;
Polymerase chain reaction
- From:
International Journal of Traditional Chinese Medicine
2025;47(12):1726-1731
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the identification of Qianghuo (Notopterygii Rhizoma et Radix) dispensing granules with PCR for the identification of dispensing granules.Methods:The collected samples were identified by DNA bar code, and the methods of extracting genomic DNA from Notopterygii Rhizoma et Radix, Qianghuo standard decoction and dispensing granules were established. Specific differential primers were designed based on ITS2 sequence, and the PCR amplification system and reaction conditions were optimized.Results:The PCR amplification system and reaction conditions were determined. The target bands of about 216 bp were obtained by amplification of Notopterygii Rhizoma et Radix, Qianghuo standard decoction and dispensing granules, and there was no interference from counterfeit products and blank.Conclusions:The specific PCR identification method of Qianghuo (Notopterygii Rhizoma et Radix) dispensing granules is established, which has good specificity. The detection limit of Notopterygii Rhizoma et Radix is 0.91 ng, and the detection limit of dispensing granules is 7.36 ng, which also provides a reference for the identification of other kinds of TCM dispensing granules.
