Effects of Tongxin Huoqian Decoction on fibrosis in mice with adenomyosis model
10.3760/cma.j.cn115398-20250514-00156
- VernacularTitle:通脉化癥汤对子宫腺肌病模型小鼠纤维化的影响
- Author:
Yinuo CHEN
1
;
Yinuo ZHANG
;
Xin WANG
;
Zilu WANG
;
Yu ZHANG
;
Wei SHI
;
Weipin NIU
Author Information
1. 山东中医药大学第一临床医学院，济南 250355
- Keywords:
Adenomyosis;
Fibrosis;
Tongmai Huazheng Decoction;
Collagen;
Fibronectin;
Transforming growth factor-beta;
α-Smooth muscle actin;
Mice
- From:
International Journal of Traditional Chinese Medicine
2025;47(12):1687-1694
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Tongmai Huazheng Decoction on the fibrosis of lesions in mice with adenomyosis (AM); To explore its mechanism.Methods:The ICR mouse model of adenomyosis was established by using tamoxifen modeling method. The 30 mice successfully modeled were divided into model group, Tongmai Huazheng Decoction group and progesterone group according to the random number table method with 10 mice in each group, and 10 female mice were set as the blank group. Tongmai Huazheng Decoction group was intragastrically administered with Tongmai Huazheng Decoction 30.47 g/kg, once/d; The gestrinone group was intragastrically administered with gestrinone suspension 0.32 mg/kg, twice a week; the blank group and model group were intragastrically administered with 0.1 ml/10 g normal saline, once a day. After 8 weeks of intervention, HE staining was used to observe the pathological changes of uterine tissue; Masson staining was used to observe the degree of uterine tissue fibrosis; immunofluorescence method was used to detect the protein expressions of CollagenⅠ, CollagenⅢ and fibronectin in uterine tissue of each group; Western bolt method was used to detect the protein expressions of TGF-β1 and α-smooth muscle actin (α-SMA) in each group.Results:Compared with the model group, the ratio of collagen fiber area in uterine tissue in Tongmai Huazheng Decoction group and progesterone group decreased ( P<0.05), the immunofluorescence intensity of CollagenⅠ, CollagenⅢ and Fibronectin decreased ( P<0.05), and the expressions of TGF-β1 and α-SMA proteins decreased ( P<0.05). Conclusion:Tongmai Huazheng Decoction can reduce the ratio of collagen fiber area in the uterus of mice with adenomyosis model, downregulate the expression of CollagenⅠ, Collagen Ⅲ, Fibronectin, TGF-β1, and α-SMA proteins, alleviate the degree of fibrosis in adenomyosis lesions, and thus inhibit or slow down disease progression.
