Study on rapid identification of Cremastrae Pseudobulbus Pleiones Pseudobulbus and its adulterants with Fourier exchange infrared spectroscopy
10.3760/cma.j.cn115398-20250218-00239
- VernacularTitle:傅里叶交换红外光谱法快速鉴别山慈菇及其混伪品研究
- Author:
Yu XUE
1
;
Huqiang FANG
;
Tao XIE
;
Jia SONG
;
Shimao CHEN
;
Zixuan JIANG
;
Wangxing CHENG
;
Qingshan YANG
;
Tangyi PENG
Author Information
1. 安徽中医药大学药学院，合肥 230012
- Keywords:
Cremastrae Pseudobulbus Pleiones Pseudobulbus;
Traditional Chinese Drug Adulteration;
FT-IR;
Second derivative;
Chemometrics
- From:
International Journal of Traditional Chinese Medicine
2025;47(11):1574-1580
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a Fourier transform infrared spectroscopy (FT-IR) method for rapid identification of Cremastrae Pseudobulbus Pleiones Pseudobulbus and its adulterants.Methods:The Fourier transform infrared spectra of Cremastrae Pseudobulbus Pleiones Pseudobulbus and its adulterants were established, and the second derivative spectral analysis, clustering analysis, principal component analysis, opls-da and cluster independent soft mode classification model were analyzed to explore the difference characteristic peaks of Cremastrae Pseudobulbus Pleiones Pseudobulbus and its adulterants.Results:The first order infrared spectrum showed that the peak shape and peak intensity of Cremastrae Pseudobulbus Pleiones Pseudobulbus and its adulterants were different. Clustering analysis, principal component analysis and OPLS-DA results showed that Cremastrae Pseudobulbus Pleiones Pseudobulbus and its adulterants showed good clustering characteristics. SIMCA method was used to construct the model, and the accuracy of the training set and the verification set were 100%, which further verified the feasibility of this method in identifying the authenticity of Cremastrae Pseudobulbus Pleiones Pseudobulbus.Conclusions:The second-order infrared spectroscopy can accurately distinguish the differences between Cremastrae Pseudobulbus Pleiones Pseudobulbus and its adulterants. The method is fast and accurate, and can be used for the authenticity identification of Cremastrae Pseudobulbus Pleiones Pseudobulbus.
