Quality difference evaluation of Anemones Raddeanae Rhizoma from different origins based on multi-index quantitative determination, chemometrics and weighted TOPSIS method
10.3760/cma.j.cn115398-20250214-00170
- VernacularTitle:基于多指标定量、化学计量学及加权TOPSIS法的不同产地两头尖质量差异评价
- Author:
Yao FU
1
;
Cheng QIAN
;
Fang LIANG
;
Cheng GUO
Author Information
1. 上海交通大学医学院附属第六人民医院药剂科，上海 200233
- Keywords:
Anemones Raddeanae Rhizoma (TCD);
High performance liquid chromatography;
Chemometrics;
Weighted TOPSIS;
Comprehensive evaluation
- From:
International Journal of Traditional Chinese Medicine
2025;47(10):1440-1447
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quality evaluation model of chemometrics and weighted TOPSIS method; To comprehensively evaluate the quality of Anemones Raddeanae Rhizoma from different origins; To provide references for further research.Methods:The simultaneous determination of the contents of leonloside D, raddeanoside R16, raddeanoside R8, hederacolchiside E, hederasaponin B, raddeanin A, betulinic acid, oleanolic acid, betulin, daucosterol, coumarin and 4,7-dimethoxyl-5-methyl-coumarin in 18 batches of Anemones Raddeanae Rhizoma were determined by HPLC. The contents of alcohol-soluble extract, total ash and acid-insoluble ash were determined according to the Anemones Raddeanae Rhizoma test items in the 2020 edition of Chinese Pharmacopoeia. Based on this, a quality evaluation model combining chemometrics and weighted TOPSIS model was constructed.Results:An Anemones Raddeanae Rhizoma multi-index quantitative methodology was established, and the results were good. There were differences in the quality of 18 batches of samples. Chemometrics could distinguish the quality of Anemones raddeanae rhizoma from different origins, and 18 batches of samples were clustered into three categories. raddeanoside R16, raddeanin A, hederasaponin B, betulinic acid, oleanolic acid and coumarin were quality difference factors. The analysis results of weighted TOPSIS method showed that the optimal Ci of weighted TOPSIS were 0.113 6-0.732 9, and the quality of the Anemones Raddeanae Rhizoma from Liaoning provinces was better.Conclusions:Multi-index quantification provides a scientific and practical new method for the quality control of Anemones Raddeanae Rhizoma medicinal materials. Chemometrics and weighted TIPSIS methods provide a basis for the quality evaluation of Anemones raddeanae rhizoma medicinal materials from different origins.
