Study on establishment of UPLC fingerprint and determination of 12 components in Aiye standard decoction
10.3760/cma.j.cn115398-20240928-00325
- VernacularTitle:艾叶标准汤剂超高效液相色谱指纹图谱建立及12个成分含量测定研究
- Author:
Yifei MA
1
;
Xiangyuan ZHOU
;
Yuanyuan XIE
;
Zhenyu LI
;
Minyou HE
;
Yong LIU
;
Wenhui LUO
;
Xian QIU
;
Qiong LUO
;
Roushan CHEN
;
Xiangdong CHEN
;
Dongmei SUN
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Artemisiae Argyi Folium;
Standard decoction;
Fingerprint;
Content determination;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2025;47(10):1425-1431
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quality control method for Aiye standard decoction.Methods:The ultra performance liquid chromatogrphy (UPLC) column Waters ACQUITY HSS T3 C18 (2.1 mm×150 mm,1.8 μm) was used to gradient elution by acetonitrile and 0.1% formic acid in water. 16 batches of Aiye standard decoction fingerprints were established by UPLC and the common peaks were determined in the fingerprints. The contents of 12 components were determined. The 16 batches of Aiye standard decoction were analyzed by similarity calculation, hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) for analysis of differential components of Artemisiae Argyi Folium from different origins.Results:A total of 13 common peaks were marked in the fingerprints of 16 batches of Aiye standard decoction, 12 of which were identified by comparison with reference substance, including chlorogenic acid, sochlorogenic acid A, neochlorogenic acid, cryptochlorogenic acid, caffeic acid,1,3-O-Dicaffeoylquinic acid, schaftoside, isochlorogenic acid B,1,5-O-Dicaffeoylquinic acid, isochlorogenic acid C, jaceosidin and eupatilin. Similarity evaluation, PCA and HCA all classified the 16 batches of Aiye standard decoction into 2 categories. Orthogonal partial least squares discriminant analysis screened 5 differential biomarkers from 13 common peaks. The content determination results showed that the phenolic compounds and flavonoids in samples from Hubei were significantly higher than that in samples from other areas.Conclusion:This method can effectively analyze the differences in the quality of Aiye standard decoction from different origins, and provide reference for the formulation of quality standards for Aiye standard decoction and related preparations.
