Exploration on the effects of Yishen Tonglong Granules on cell proliferation and apoptosis in nude mice with androgen independent prostate cancer based on non classical Wnt signaling pathway
10.3760/cma.j.cn115398-20250108-00101
- VernacularTitle:基于非经典Wnt信号通路探究益肾通癃汤对雄激素非依赖性前列腺癌荷瘤裸鼠细胞增殖与凋亡的影响
- Author:
Wenxiong ZHU
1
;
Wenjing XU
;
Xi ZHANG
;
Yifeng YUAN
;
Qihua CHEN
;
Guozheng QIN
Author Information
1. 湖南中医药大学第一附属医院男科，长沙 410007
- Keywords:
Wnt signaling pathway;
Yishen Tonglong Granules;
PC-3 cells;
Prostatic neoplasms;
Nude mice bearing tumor
- From:
International Journal of Traditional Chinese Medicine
2025;47(10):1404-1411
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of Yishen Tonglong Granules (YSTLG) on cell proliferation and apoptosis in a nude mouse model of androgen independent prostate cancer subcutaneous transplantation based on non classical Wnt signaling pathway.Methods:The tumor-bearing nude mouse model was established using the human prostate cancer bone metastatic cell line PC-3. After successful modeling, the mice were equally divided into six groups using the random number table method: model group, Western medicine group, Chinese materia medica low-, medium-, and high-dosage groups, and Chinese materia medica-Western medicine combination group. Corresponding drug interventions were administered to each group. Following 28 consecutive days of drug administration, the nude mice were euthanized. Tumor tissues were harvested for pathological observation via HE staining. Cell proliferation was assessed by immunohistochemistry; apoptosis was detected using TUNEL assay; the expressions of non-canonical Wnt signaling pathway-related proteins (Wnt5a, Rac1, RhoA, NFATc1) were analyzed through Western blot and immunohistochemical methods.Results:THE staining results demonstrated that YSTLG could effectively ameliorate pathological alterations in tumor tissues. Compared with the model group, the Chinese materia medica low-, medium-, and high-dosage groups, as well as the Chinese materia medica-Western medicine combination group, exhibited reduced proliferation indices ( P<0.01), elevated apoptosis indices ( P<0.01), down-regulated protein expressions of Wnt5a, Rac1, RhoA, and NFATc1 ( P<0.01), and decreased optical density values of Wnt5a, Rac1, RhoA, and NFATc1 ( P<0.01). These effects displayed a dosage-dependent trend. The Chinese materia medica-Western medicine combination group achieved the most pronounced therapeutic outcomes. Conclusions:YSTLG may exert inhibitory effects on the proliferation of androgen-independent prostate cancer cells and promote apoptosis, possibly through suppression of the non-canonical Wnt signaling pathway. Furthermore, its combination with Wnt signaling inhibitors may exhibit synergistic therapeutic effects.
