Study on quality standard of wine-processed Coptidis Rhizoma standard decoction
10.3760/cma.j.cn115398-20241211-00112
- VernacularTitle:酒黄连标准汤剂质量标准研究
- Author:
Huilin YANG
1
;
Kaiwei HUANG
;
Yanghua LI
;
Suqin CAI
;
Shuping XU
;
Jiabao WEI
;
Hui ZHANG
;
Weizhi ZHAO
;
Pei TAN
Author Information
1. 华润三九现代中药制药有限公司，惠州 516000
- Keywords:
Coptidis Rhizoma (TCD);
Processing (TCD);
Standard Decoction;
Fingerprinting;
Content determination;
High performance liquid chromatography
- From:
International Journal of Traditional Chinese Medicine
2025;47(9):1285-1292
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality standard of the standard decoction of wine-processed Coptidis Rhizoma by studying the extraction rate, fingerprint and component quantitative analysis.Methods:ccording to the Technical Requirements for Quality Control and Standard Formulation of Chinese Medicine Formula Granules, 15 batches of the standard decoction of wine-processed Coptidis Rhizoma were prepared, and the paste rate was determined; HPLC fingerprints of 15 batches of standard decoction of wine-processed Coptidis Rhizoma were established, and evaluated by combining similarity evaluation, clustering analysis, principal component analysis and orthogonal partial least squares discriminant analysis; the contents of berberine, epiberberine, pamadine, and safranine in the samples of the 15 batches were determined and analyzed their transfer rates.Results:A total of 15 batches of standard decoction samples were calibrated with 11 common peaks, referring to the recognition of 8 components. The similarity between the samples and the control product was greater than 0.900; the clustering analysis could cluster the 15 batches of samples into 2 classes; the results of the principal component analysis showed that the cumulative variance contribution rate of the 3 principal component factors was 89.388%; the OPLS-DA screened out the 3 components of the quality difference; the 15 batches of samples out of the paste rate was 15.7% -20.8%, and the mass fractions of berberine, epiberberine, safranine, and palmatine were 18.47%-24.38%, 2.82%-3.49%, 5.08%-6.69%, and 4.84%-6.68%, respectively, with transfer rates of 41.7%-61.7%, 46.9%-68.7%, 39.8%-61.5%, and 43.8%-65.2%.Conclusion:The fingerprint and content determination method established in this study is accurate, stable, simple, and can be used for the quality control and evaluation of the standard decoction of wine-processed Coptidis Rhizoma.
