Evaluation of the quality of Su Pinelliae Rhizoma by combining fingerprint spectroscopy with chemometrics and multi-component analysis
10.3760/cma.j.cn115398-20250217-00216
- VernacularTitle:指纹图谱结合化学计量学和多成分测定评价宿半夏质量
- Author:
Xiaoming YUAN
1
;
Chong HU
;
Jingzhe PU
Author Information
1. 安徽省宿州市食品药品检验检测中心，宿州 234000
- Keywords:
Pinelliae Rhizoma;
Fingerprint;
Chemometrics;
Content determination;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2025;47(9):1277-1284
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish fingerprint spectra of Pinelliae Rhizoma from different production areas; To comprehensively evaluate the quality of Pinelliae Rhizoma and Su Pinelliae Rhizoma from other main producing areas by screening and content determination of different components combined with chemometrics.Methods:Shimadzu Shim pack GIST C18-AQ (250 mm ×4.6 mm, 5 μm) chromatography column was used, with acetonitrile aqueous solution as the mobile phase, gradient elution, flow rate of 0.8 ml/min, detection wavelength of 260 nm, column temperature of 25 ℃, and injection volume of 20 μl. Chemometric analysis were performed using SIMCA 14.1 and SPSS 22.0 software. Heterogeneous indicator components were screened out basing on a VIP value greater than 1. Some components were identified and determined the contents. Three principal components were extract basing on the characteristic value λ>1 and cumulative variance contribution rate greater than 85%. Linear weighted sum was performed by variance contribution rate. The samples were calculated the comprehensive score and ranked to evaluated the overall quality.Results:There were a total of 11 common peaks in the fingerprint spectra of 16 batches of Pinelliae Rhizoma, and the results of methodological investigation met the technical requirements of fingerprints, with a similarity of 0.623-0.975. 5 chromatographic peaks were identified, which were cytidine (peak 3), uridine (peak 4), adenine (peak 5), guanosine (peak 6) and adenosine (peak 8), respectively. 16 batches of Pinelliae Rhizoma were grouped into 2 categories: S1, S4, S5, S7, S9-S18 were grouped into one category, and S2, S3, S6, and S8 batches of Su Pinelliae Rhizoma were grouped into another. The comprehensive score showed that the overall quality of wild Pinelliae Rhizoma in Dangshan, wild Pinelliae Rhizoma in Zhijin, Guizhou, and Pinelliae Rhizoma in Xihe, Gansu was good. Seven differential components with VIP values greater than 1 were screened out, and the active contents of uridine, guanosine and adenosine were determined. The results showed that in 5 batches of Su Pinelliae Rhizoma (S2, S3, S6, S7, S8) samples, the total amount of uridine, guanosine and adenosine(0.450-1.177 mg/g) was significantly higher than that of Pinelliae Rhizoma from other regions, especially the Su Pinelliae Rhizoma planted in Suixi County.Conclusions:The fingerprint spectrum combined with chemometrics and content determination of differential component is a stable and reliable method for evaluating the quality of Pinelliae Rhizoma from different regions. It can provide references for the overall quality control and evaluation of Pinelliae Rhizoma, as well as research on artificial cultivation and introduction of Su Pinelliae Rhizoma.
