Study on quality evaluation of Alpiniae Katsumadai Semen based on HPLC specific chromatogram and chemometricsy
10.3760/cma.j.cn115398-20241118-00222
- VernacularTitle:基于HPLC特征图谱及化学计量学的草豆蔻质量评价研究
- Author:
Xiangyuan ZHOU
1
;
Jiao PENG
;
Siqiong CAO
;
Peiqi LUO
;
Xiangdong CHEN
;
Dongmei SUN
;
Zhenyu LI
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Katsumada Galangal Seed (TCD);
Chemometrics;
Specific chromatogram;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2025;47(9):1272-1276
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the contents of four flavonoids by establishing HPLC specific chromatogram for Alpiniae Katsumadai Semen; To evaluate the differences of Alpiniae Katsumadai Semen from different producing areas.Methods:The specific chromatogram was developed on a column of Thermo Acclaim C18 with acetonitrile-0.1% phosphoric acid solution as the mobile phase by gradient elution at a flow rate of 1.0 ml/min. The detective wavelength was 260 nm, and the column temperature was 30 ℃. Similarity evaluation, PCA analysis, and OPLS-DA analysis were conducted. The contents of Alpinetin, Pinocembrin, Cardamonin, Alnustone in 16 batches of Alpiniae Katsumadai Semen.Results:There were 9 characteristic peaks in the specific chromatogram of Alpiniae Katsumadai Semen. Except the sample of S2 (Hainan producing area), the similarity of Alpiniae Katsumadai Semen in different producing areas was greater than 0.90; PCA analysis divided 16 batches of Alpiniae Katsumadai Semen into 2 categories, and OPLS-DA analysis identified 4 differential biomarkers, with the order of impact being peak 3>peak 5>Alpinetin>Cardamonin. Among them, the quality of Alpiniae Katsumadai Semen from Guangdong producing area was generally stable. Moreover, there were significant differences in the contents of Alpinetin and Cardamonin among the indicator components of Alpiniae Katsumadai Semen from different producing areas.Conclusion:This method can effectively analyze the differences in the quality of Alpiniae Katsumadai Semen from different producing areas, providing reference for the quality evaluation of Alpiniae Katsumadai Semen.
