Establishment of UPLC characteristic spectrum and quantitative analysis of piperine for Hujiao standard decoction
10.3760/cma.j.cn115398-20241210-00099
- VernacularTitle:胡椒标准汤剂UPLC特征图谱建立及胡椒碱定量分析
- Author:
Shuangyan TANG
1
;
Jiabao WEI
;
Zhihong ZHAN
;
Yidan TONG
;
Jiahui XIE
;
Hui ZHANG
Author Information
1. 华润三九现代中药制药有限公司，惠州 516000
- Keywords:
Piperis Fructus (TCD);
Standard decoction;
Piperine;
Characteristic map;
Entropy weight method;
Grey relational analysis
- From:
International Journal of Traditional Chinese Medicine
2025;47(8):1134-1140
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish UPLC characteristic spectrum of Hujiao standard decoction and the determination method for the content of piperine.Methods:15 batches of freeze-dried powder of Hujiao standard decoction were prepared according to the traditional decocting method. The range of paste yield was determined, and the UPLC characteristic spectrum of the standard decoction was established. High-resolution mass spectrometry and control products were used to identify common peaks. Based on the common peak area, the weights of each peak were compared using entropy weight method, and correlation analysis and similarity evaluation were conducted using clustering analysis and grey correlation method; a method for determining the content of piperine in Hujiao decoction pieces and freeze-dried powder of standard decoction was simultaneously established, and the transfer rate was calculated.Results:The extraction rate of 15 batches of freeze-dried powder of Hujiao standard decoction ranged from 10.4% to 16.8%, with an average of 14.0%. The content of piperine ranged from 12.2 to 30.0 mg/g, with an average of 18.5 mg/g, and the transfer rate ranged from 4.0% to 7.8%, with an average of 5.8%. Six common peaks were identified in the established characteristic spectrum and identified by high-resolution mass spectrometry and control products respectively. Peak 1 was N-trans-feruloyltyramine, peak 3 was piperine and the similarity was 0.959-1.000. Clustering analysis and grey correlation analysis showed that there was little difference between quality of Piperis Fructus and origins.Conclusion:In this study, the characteristic spectrum and content determination method of freeze-dried powder of Hujiao standard decoction are established, which can provide references for quality detection and control of Hujiao standard decoction or its derivative products.
