Analysis on the quality of Viticis Fructus and its processed products based on fingerprint combining multivariate statistical method and component difference analysis
10.3760/cma.j.cn115398-20241220-00232
- VernacularTitle:基于指纹图谱结合多元统计方法和成分差异分析蔓荆子及其炮制品质量
- Author:
Minyou HE
1
;
Liwei WANG
;
Hongxing PENG
;
Xinya WAN
;
Poyu ZHANG
;
Xiangdong CHEN
;
Dongmei SUN
;
Congyou DENG
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Viticis Fructus;
Fried Viticis Fructus;
Fingerprints;
Multivariate statistical analysis;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2025;47(8):1119-1126
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the UPLC fingerprint evaluation system of Viticis Fructus; To comprehensively evaluate the quality of Viticis Fructus and its processed products combining with multivariate statistical methods and compositional variance analysis.Methods:19 batches of Viticis Fructus from different regions were collected and processed by frying process into decoction pieces. The separation was operated on Waters CORTECS T3 C18 chromatographic column (100 mm × 2.1 mm, 1.6 μm). Acetonitrile and 0.1% phosphoric acid water were used as mobile phases for gradient elution, to establish the UPLC fingerprints of Viticis Fructus. The UPLC fingerprints of Viticis Fructus were analyzed using similarity evaluation, principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA). The contents of seven active components in the samples of Viticis Fructus and fried Viticis Fructus were determined.Results:A total of 26 common peaks were identified in the fingerprints of 38 batches of samples, and 7 components were identified. Similarity evaluation results demonstrated that the chemical components of Viticis Fructus from Jingdezhen City, Jiangxi Province, were significantly different from those of other regions. The results of PCA and PLS-DA analysis showed that the chemical components of Viticis Fructus and fried Viticis Fructus could be clearly distinguished, and the processing process had an impact on the components. The results of content determination showed that the contents of some components increased or decreased after frying. The analysis results of grey correlation method and TOPSIS method show that the medicinal materials in Jiujiang City, Jiangxi Province have a high score ranking and stable quality.Conclusion:This study successfully establishes the fingerprints of Viticis Fructus and its processed products, grey correlation method and TOPSIS method analysis revealed the quality differences of samples from different origins, which providing a scientific basis for the quality control and evaluation of Viticis Fructus and its processed products.
