Discussion on the effects of Shenshuaikang Enema on H/ R-induced senescence of NRK-52E cells based on P53/P21 pathway
10.3760/cma.j.cn115398-20241210-00098
- VernacularTitle:基于P53/P21通路探讨肾衰康灌肠液对缺氧复氧NRK-52E细胞衰老的影响
- Author:
Yu ZHANG
1
;
Lizeyu LYU
;
Yong LIN
;
Lihua WU
;
Mengfan YANG
;
Anqi TANG
;
Mingquan LI
Author Information
1. 成都中医药大学临床医学院，成都 610000
- Keywords:
Cellular senescence;
Shenshuaikang enema;
Tumor suppressor protein p53;
Cell cycle;
Renal tubular epithelial cell repair
- From:
International Journal of Traditional Chinese Medicine
2025;47(8):1103-1109
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and mechanism of Shenshuaikang Enema on hypoxia/reoxygenation (H/R) NRK-52E cells; To provide references for Shenshuaikang Enema to treat AKI.Methods:The H/R-induced NRK-52E cell model was established, and control group, model group, drug-containing serum group, drug-containing blood group +p53 agonist group, p53 agonist group, p53 inhibitor group were set up. Cell viability was detected by CCK8. The cell cycle distribution in each group was analyzed using flow cytometry, while cell senescence was assessed via β-galactosidase staining. The levels of IL-6, IL-1β, and TNF-α in the cell supernatant were evaluated using ELISA. Western Blot analyses were conducted to measure the protein expressions of p53, phosphorylated p53 (p-p53), and p21.Results:Compared with model group, NRK-52E cell vitality significantly increased in the drug-containing serum group and p53 inhibitor group ( P<0.01, P<0.05), S phase and G2/M phase percentage was significantly reduced ( P<0.01), β-galactoase staining decreased ( P<0.01), the levels of IL-1β, IL-6 and TNF-α decreased ( P<0.05, P<0.01), the protein expressions of p-p53 and p21 decreased ( P<0.01). Compared with the drug-containing serum group, NRK-52E cell vitality significantly decreased in the drug-containing serum+p53 agonist group and p53 agonist group ( P<0.01), S phase and G2/M phase percentage was significantly increased ( P<0.01), β-galactoase staining increased ( P<0.01), the levels of IL-1β, IL-6 and TNF-α increased ( P<0.01), the protein expressions of p-p53 and p21 increased ( P<0.01 or P<0.05). Conclusion:The drug-containing serum of Shenshuaikang Enema may promote cell proliferation, improve cell cycle arrest, inhibit pro-inflammatory and senescence related secretory phenotypes, and inhibit cell senescence by inhibiting p53/p21 signaling pathway, so as to promote H/ R-induced NRK-52E cell damage repair.
