Effects of Huaiqihuang Granules on airway remodeling and immune function in asthmatic young rats through IL36/lL36R/MyD88 signaling pathway
10.3760/cma.j.cn115398-20241009-00052
- VernacularTitle:槐杞黄颗粒通过IL36/lL36R/MyD88通路对哮喘幼鼠气道重塑及免疫功能的影响
- Author:
Heqing HUANG
1
;
Ying CHEN
Author Information
1. 武汉市东西湖区人民医院（华中科技大学协和东西湖医院）儿科，武汉 430040
- Keywords:
Asthma;
Huaiqihuang granules;
Airway remodeling;
Immune function;
Inflammation
- From:
International Journal of Traditional Chinese Medicine
2025;47(8):1089-1094
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Huaiqihuang Granules on airway remodeling and immune function in asthmatic young rats through IL36/lL36R/MyD88 signaling pathway.Methods:The young mice were divided into normal group, model group, Chinese materia medica low-, medium-, and high-dosage groups using a random number table method, with 10 mice in each group. Except for the normal group, asthma models were established in all other groups. Chinese materia medica low-, medium-, and high-dosage groups were orally administered with 2, 4, and 8 g/kg Huaiqihuang Granules, respectively. The normal group and model group were orally administered with an equal volume of physiological saline once a day for 2 consecutive weeks of intervention. Pulmonary function apparatus was used to detect inspiratory resistance (Ri), expiratory resistance (Re) and dynamic compliance (Cdyn) of the pups; flow cytometry was used to detect interleukin 2/4 (IL-2, IL-4), interferon (IFN-γ) and immunoglobulin E (IgE) in peripheral blood; HE staining was used to observe the changes of lung tissue cases of the pups; Western blotting was used to detect the protein expressions of IL-36, IL-36R and MyD88.Results:Compared with the model group, the Chinese materia medica medium- and high-dosage groups showed a decrease in Ri and Re ( P<0.05), and an increase in Cdyn ( P<0.05); the thickness of airway wall and the number of smooth muscle cells decreased ( P<0.05); the levels of IL-4 and IgE in peripheral blood decreased ( P<0.05), while the levels of IFN-γ and IL-2 increased ( P<0.05); the expressions of IL-36, IL-36R, and MyD88 proteins in lung tissue decreased ( P<0.05), and there was a certain dosage-dependent relationship among the Chinese materia medica low-, medium-, and high-dosage groups ( P<0.05). The HE staining results showed that the airway and lung tissues of the model group mice were accompanied by a large number of inflammatory pathological changes. The alveolar cavities and inflammatory infiltration of the mice in each treatment group were improved, with the Chinese materia medica high-dosage group showing the most significant improvement. Conclusion:Huaiqihuang Granules can improve airway remodeling and immune function in asthmatic model mice, and its mechanism may be related to regulating IL36/lL36R/MyD88 signaling pathway, improving airway remodeling and reducing inflammatory response.
