Study on protective effect of Jinbuhuan Jianwei Jiedu Decoction regulating bile acid FXR/TGR5 pathway on gastric mucosa of CAG rats improving airway remodeling and reducing inflammatory response
10.3760/cma.j.cn115398-20241030-00324
- VernacularTitle:金不换健胃解毒汤调节胆汁酸FXR/TGR5途径对慢性萎缩性胃炎大鼠胃黏膜的保护作用研究
- Author:
Ling ZHANG
1
;
Yubin CHEN
;
Zhangkai YANG
;
Yipei WANG
Author Information
1. 广东省中医院海南医院脾胃病科，海口 570311
- Keywords:
Gastritis, atrophic;
Jinbuhuan Jianwei Jiedu Decoction;
Bile acids;
FXR/TGR5 pathway;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(8):1082-1088
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the protective effect of Jinbuhuan Jianwei Jiedu Decoction on gastric mucosa of rats with chronic atrophic gastritis (CAG); To explore its mechanism through the farnesyl ester X receptor (FXR)/G protein bile acid coupled receptor 5 (TGR5) pathway.Methods:Wistar rats were randomly divided into normal control group, model group, folic acid group, low-, medium-, and high-dosage groups, with 10 rats in each group. Except for the normal control group, the other rats were used to establish a CAG model by alternate gavage of 2% sodium salicylate+20 mmol/L sodium deoxycholate+methylnitrosoguanidine (MNNG) for free consumption. The folic acid group was treated with 1.43 mg/kg by gavage, while the low-, medium-, and high-dosage groups were treated with 9.68, 19.35 and 38.7 g/kg of Jinbuhuan Jianwei Jiedu Decoction by gavage, once a day, for 12 weeks. The gastric mucosal blood flow and histopathological changes in each group of rats were observed. Rat gastric juice was collected, and gastric juice pH and pepsin activity were detected. ELISA method was used to detect the levels of serum tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and total bile acid (TBA). Western-blot and RT-PCR were used to detect the protein and mRNA levels of FXR, TGR5, CDX2 and SOX2 in gastric mucosal tissue.Results:Compared with the normal control group, the gastric mucosal blood flow and pepsin activity in the low-, medium-, and high-dosage groups increased significantly, and the pH value of gastric juice of medium-, and high-dosage groups decreased ( P<0.05). The levels of TNF-α, IL-6, TBA in serum of low-, medium-, and high-dosage groups decreased ( P<0.05); and FXR, TGR5, CDX2 in gastric mucosal tissue decreased, while the protein expression of SOX2 increased ( P<0.05). Conclusion:Jinbuhuan Jianwei Jiedu Decoction has a repairing effect on gastric function and mucosal lesions in CAG rats. Its mechanism may be related to the inhibition of abnormal activation of FXR/TGR5 pathway, regulation of bile acid and inflammatory mediator secretion.
