Effects of modified Chaihu Jia Longgu Muli Decoction on the efficacy of Parkinson's disease with liver and kidney yin deficiency syndrome and the phosphatase and tensin homolog-induced putative kinase 1 (PINK1)/E3 ubiquitin ligase (Parkin) signaling pathway induced by phosphatase and tensin homologues
10.3760/cma.j.cn115398-20240923-00245
- VernacularTitle:柴胡加龙骨牡蛎汤加减对帕金森病肝肾阴虚证疗效及其同源性磷酸酶张力蛋白诱导激酶1/E3泛素连接酶信号通路的影响
- Author:
Qingping REN
1
;
Hai LIN
;
Chen WANG
;
Chunmei YANG
;
Kaina WANG
;
Min LI
Author Information
1. 西安市中医医院脑病科，西安 710021
- Keywords:
Parkinson disease;
Chaihu Jia Longgu Oyster Soup;
Liver and kidney yin deficiency;
PINK induced protein kinase 1;
E3 ubiquitin ligase
- From:
International Journal of Traditional Chinese Medicine
2025;47(8):1066-1071
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical efficacy of modified Chaihu Jia Longgu Muli Decoction in patients with liver and kidney Yin deficiency syndrome of Parkinson's disease (PD) and the effects on phosphatase and tensin homolog-induced putative kinase 1 (PINK1) /E3 ubiquitin ligase (Parkin) signaling pathway.Methods:A randomized controlled trial study was conducted. A total of 120 PD patients from the Department of Encephalopathy of Xi'an Hospital of Traditional Chinese Medicine from May 2020 to May 2024 were selected as the observation objects and divided into 2 groups through random number table method, with 60 cases in each group. The control group took dopashydrazine tablets orally, while the combined group took Chaihu Jia Longgu Muli Decoction on the basis of the control group. Two groups were treated continuously for 2 months. The levels of glial cell-derived neurotrophic factor (GDNF), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and basic fibroblast growth factor 2 (FGF2) were detected by ELISA. The expression levels of PINK1, Parkin and chelate 1 (SQSTM1) mRNA were detected by real-time fluorescence quantitative PCR. The adverse reactions during the treatment were observed and recorded to evaluate the clinical efficacy.Results:The total effective rate was 95.00% (57/60) in the combined group and 83.33% (50/60) in the control group, with statistical significance ( χ2=4.23, P=0.040). After treatment, the levels of serum GDNF [(510.78±31.57) ng/L vs. (431.89±30.45) ng/L, t=13.93], BDNF [(36.47±3.72) ng/L vs. (27.84±3.55) ng/L, t=13.00], NGF [(57.24±4.17) ng/L vs. (50.72±4.11) ng/L, t=8.63] and FGF2 [(26.21±3.71) ng/L vs. (21.63±3.54) ng/L, t=6.92] in the combined group were higher than those in the control group ( P<0.001). After treatment, the mRNA expression levels of PINK1 (2.38±0.07 vs. 2.19±0.05, t=17.11), Parkin (1.89±0.03 vs. 1.74±0.05, t=19.93), and SQSTM1 (1.82±0.07 vs. 1.61±0.05, t=18.91) in the combined group were higher than those in the control group ( P<0.001). During the treatment period, the incidence of adverse reactions was 6.67% (4/60) in the combined group and 10.00% (6/60) in the control group, without statistical significance ( χ2=0.44, P=0.509). Conclusion:Modified Chaihu Jia Longgu Muli Decoction can increase the levels of neurotrophic factors in patients with PD of liver and kidney yin deficiency syndrome, regulate the PINK1/Parkin pathway, protect neurons, improve mitochondrial autophagy function, and enhance clinical efficacy.
