Analysis on effective components and biological activity of volatile oil in anti-URTI formula
10.3760/cma.j.cn115398-20240711-00122
- VernacularTitle:抗上呼吸道感染方剂挥发油有效成分分析及其生物活性研究
- Author:
Heming FAN
1
;
Yue LI
;
Tuoxin LI
;
Liying JIA
;
Pengfei GAO
;
Dongping LIU
;
Donghe HAN
;
Xian ZHANG
;
Lin ZHANG
Author Information
1. 首都医科大学附属北京中医医院研究所，北京 100010
- Keywords:
Respiratory tract infections;
Volatile oil determination (TCD);
Antioxidant;
Anti-inflammatory
- From:
International Journal of Traditional Chinese Medicine
2025;47(6):843-848
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the extraction, chemical composition, antioxidant, and anti-inflammatory activity of the TCM formula essential oil for the treatment of upper respiratory tract infections (URTI); To provide a scientific basis for its further development.Methods:The formula essential oil was extracted using the steam distillation method and analyzed for chemical composition by gas chromatography-mass spectrometry (GC-MS). The DPPH, ABTS scavenging ability, and hydroxyl radical scavenging ability of volatile oils were measured. The effect of the essential oil on the viability of RAW264.7 cells was assessed using the CCK-8 assay. ELISA and Western blot methods were used to determine the effects of volatile oil on LPS induced inflammatory cytokines IL-6 and TNF-α.Results:The average extraction rate of the formula essential oil was 1.12%, with a density of 0.973 2 g/ml. Twelve main chemical components were identified, with 1,8-cineole (42.9%) and patchoulol (19.9%) being the predominant constituents. The essential oil exhibited DPPH and ABTS radical scavenging capacities of 52% and 59%, respectively, and a hydroxyl radical scavenging capacity exceeding 70%. Essential oil could reduce the levels of IL-6 and TNF-α ( P<0.05). Conclusion:TCM formula essential oil for the treatment of URTI contains multiple bioactive components and demonstrates significant antioxidant and anti-inflammatory effects.
