Effect of Qingsui Xiaoyan Decoction on osteoclast differentiation of RAW264.7 cells
10.3760/cma.j.cn115398-20240401-00005
- VernacularTitle:清髓消炎汤对RAW264.7细胞破骨分化的影响
- Author:
Han GUO
1
;
Yanan QIAN
;
Qiuxia LYU
;
Yang LI
;
Qian CHEN
;
Yujing CAO
Author Information
1. 河南中医药大学骨伤医院，郑州 450052
- Keywords:
Osteoclast;
Qingsui Xiaoyan Decoction;
RAW264.7;
NF-κB signaling pathway
- From:
International Journal of Traditional Chinese Medicine
2025;47(6):807-812
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Effects of Qingsui Xiaoyan Decoction on osteoclast differentiation of RAW264.7 cells.Methods:Receptor activator of nuclear factor kappa B ligand (RANKL) was used to induce differentiation of RAW264.7 cells into osteoclasts. CCK-8 method was used to screen experimental drug concentrations and analyze the cytotoxicity of drug intervention on RAW264.7 cells. TRAP staining method was used to quantitatively detect the effects of Qingsui Xiaoyan Decoction on osteoclast differentiation ability. RT-PCR technology was used to detect the mRNA expressions of osteoclast differentiation genes TRAP, MMP-9, CK, CTR. Western blot was used to detect osteoclast-related protein expressions of NF-κBP65, IκB-αTRAP, and TRAP.Results:After 72 hours of intervention, 0.800, 4.000, 20.000, and 100.000 mg/ml of Qingsui Xiaoyan Decoction could inhibit cell proliferation ( P<0.05). Compared with the model group, the number of TRAP-positive cells decreased ( P<0.05) in the 5, 20, 80, and 160 μg/ml Qingsui Xiaoyan Decoction groups. Additionally, the mRNA levels of TRAP, MMP-9, CK, and CTR decreased ( P<0.05), the expression of NF-κB p65 and TRAP proteins decreased ( P<0.05), and the expression of IκB-α protein increased ( P<0.05). Conclusions:Qingsui Xiaoyan Decoction can inhibit the expressions of TRAP, MMP-9, CK, CTR genes and TRAP protein, reduce the degradation of I κ B - α protein by inhibiting the NF - κ B signaling pathway, inhibit the expression of NF - κ B p65 protein, and thus inhibit the differentiation of RAW264.7 into osteoclasts.
