Effects of Alpiniae Oxyphyllae Fructus-Linderae Radix on miR-155-5p/SOCS1/inflammatory factor axis and cell apoptosis in hippocampus of depressed rat model based on Wenbu Shenyang method
10.3760/cma.j.cn115398-20240401-00179
- VernacularTitle:基于温补肾阳法探讨益智仁-乌药对抑郁模型大鼠海马组织miR-155-5p/SOCS1/炎症因子轴及细胞凋亡的影响
- Author:
Pengwan LI
1
;
Zhu WU
;
Dehui YIN
;
Ye ZHU
Author Information
1. 海南医科大学中医学院，海口 571199
- Keywords:
Warming reinforcing kidney yang;
Depression;
Alpiniae Oxyphyllae Fructus-Linderae Radix;
miR-155-5p;
SOCS1/inflammation factor signaling pathway;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(6):784-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Alpiniae Oxyphyllae Fructus-Linderae Radix on miR-155-5p/SOCS1/inflammatory factor axis and cell apoptosis in hippocampus of depressed rats.Methods:A total of 60 SPF male Wistar rats were divided into six groups: control group, model group, fluoxetine group and Alpiniae Oxyphyllae Fructus-Linderae Radix low-, middle- and high-dosage groups, with 10 rats in each group. Except for the control group, all other groups used chronic mild unpredictable stimuli combined with solitary confinement to construct a depression rat model. Following an 8-week modeling period, the fluoxetine group received oral administration of 2 mg/kg Fluoxetine liquid daily and the Alpiniae Oxyphyllae Fructus-Linderae Radix groups received oral administration of 1 g/kg, 2 g/kg and 4 g/kg Alpiniae Oxyphyllae Fructus-Linderae Radix liquid daily, whereas the control group and model group were given an equivalent volume of distilled water for continuous drug intervention over a span of 3 weeks. The rats' behavioral variations were assessed through an open field test; miR-155-5p expression in the hippocampus was determined via RT-PCR; SOCS1 protein expression in the hippocampus was evaluated by Western Blot; TNF-α, IL-6 and IL-1β levels in the serum were quantified using ELISA; hippocampal cell apoptosis was examined through HE staining.Results:Compared with the model group, the fluoxetine group and Alpiniae Oxyphyllae Fructus-Linderae Radix middle- and high-dosage groups showed an increase in open field test scores ( P<0.05), a decrease in miR-155-5p expression in the hippocampus ( P<0.05), an increase in SOCS1 protein expression in the hippocampus ( P<0.05), a decrease in serum TNF-α, IL-1β, IL-6 levels ( P<0.05), and a reduction in apoptosis of hippocampal CA3 cells ( P<0.05). Conclusion:Alpiniae Oxyphyllae Fructus-Linderae Radix probably amplifies the indirect negative regulation of SOCS1 protein on inflammatory factors by suppressing miR-155-p expression in the hippocampus of depressed rats, thereby ameliorating cell apoptosis in the hippocampal area and manifesting antidepressant properties.
