Study on the identification of the characteristic peptides of the Galli Gigerii Endothelium Corneum crude drugs and preparations
10.3760/cma.j.cn115398-20240621-00207
- VernacularTitle:鸡内金药材及其制剂特征肽鉴别研究
- Author:
Guowei LI
1
;
Peizhen TONG
;
Qiping HU
;
Yunjing QIU
;
Liye PAN
;
Jiaying HE
;
Xiangdong CHEN
;
Dongmei SUN
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Galli Gigerii Endothelium Corneum;
Duck endothelium corneum;
Goose endothelium corneum;
Pigeon endothelium corneum;
Place of production (TCD);
Characterist
- From:
International Journal of Traditional Chinese Medicine
2025;47(5):676-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a mass spectrometry method for the determination of characteristic peptides of Galli Gigerii Endothelium Corneum that can identify the authenticity of Galli Gigerii Endothelium Corneum as well as its preparations; To evaluate their quality.Methods:Ultra performance chromatography-electrospray tandem mass spectrometry (UPLC-MS/MS) with the mode of multiple reaction monitoring quantification (MRM) was employed to monitor the ion pairs of m/z 379.21(charge: +2)→571.36, m/z 379.21(charge: +2)→385.26, m/z 785.41(charge: +2)→941.51 and m/z 785.41(charge: +2)→245.08, in order to detect the Galli Gigerii Endothelium Corneum crude drug and its preparations. Results:Chicken specific peptide I and chicken specific peptide Ⅱ could be detected in the 18 batches of Galli Gigerii Endothelium Corneum from different regions, their corresponding extractions and 4 batches of prescription preparations, while the chicken specific peptides were not detected in the 8 batches of endothelium corneums from ducks, geese and pigeons.Conclusions:The method established in this study can effectively supplement the deficiencies in standards of Galli Gigerii Endothelium Corneum and its decoction pieces, improve the quality control standard, and provide a reference for the safety and effectiveness of Galli Gigerii Endothelium Corneum in clinical medication.
