Establishment of UPLC characteristic spectrum of Liushenqu standard decoction and determination of related index components
10.3760/cma.j.cn115398-20241017-00161
- VernacularTitle:六神曲标准汤剂UPLC特征图谱建立及相关指标成分含量测定
- Author:
Jiahui XIE
1
;
Jiabao WEI
;
Shuangyan TANG
;
Kaiwei HUANG
;
Weizhi ZHAO
;
Yu HU
;
Hui ZHANG
Author Information
1. 华润三九现代中药制药有限公司，惠州 516000
- Keywords:
Liu shen qu tablet;
Standard decoction;
Ultra-high performance liquid chromatography;
Characteristic map;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2025;47(5):669-675
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the characteristic spectrum of Liushenqu standard decoction using ultra-high performance liquid chromatography (UPLC); To determine the contents of related index components; To evaluate the quality of Liushenqu standard decoction.Methods:UPLC method was used to establish characteristic spectrum of Liushenqu standard decoction. Chromatographic Fingerprint Similarity Evaluation System (2012 edition) was used for similarity analysis, the characteristic peak was assigned, and the content of its index components was determined.Results:The characteristic peaks of Liushenqu standard decoction were calibrated and 8 components were identified, namely uridine, adenosine, guanosine, 5-hydroxymethylfurfural, tryptophan, vanillic acid, ferulic acid and shaftaside. The contents of uridine, adenosine, tryptophan ferulic acid and shaftaside in 10 batches of Liushenqu standard decoction were simultaneously determined, and ranged from 0.036 1～0.383 9 mg/g, 0.030 7～0.170 2 mg/g, 0.007 0～0.060 2 mg/g, 0.001 0～0.005 0 mg/g, 0.000 8～0.013 8 mg/g, respectively. The transfer rates ranged from 44.2% to 50.8%, 60.1% to 67.7%, 60.4% to 76.4%, 62.7% to 77.4%, 50.7% to 61.4%, respectively.Conclusion:The established UPLC characteristic spectrum and content determination method are accurate and repeatable, which can provide references for quality control of Liushenqu standard granules.
