Effects of ginseng polysaccharide on myocardial fibrosis and endothelial function in coronary heart disease model rats based on MEK/ERK signaling pathways
10.3760/cma.j.cn115398-20241017-00167
- VernacularTitle:基于MEK/ERK信号通路探讨人参多糖对冠心病模型大鼠心肌纤维化和内皮功能的影响
- Author:
Yunpeng WU
1
;
Yisheng WANG
Author Information
1. 上海市中医医院中医康复科、心病科，上海 200071
- Keywords:
Panaxan;
Coronary heart disease;
MEK/ERK signaling pathway;
Myocardial fibrosis;
Endothelial function;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(5):638-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effects and mechanisms of ginseng polysaccharide in myocardial fibrosis and endothelial function in coronary heart disease model rats based on mitogen activated extracellular signal regulated kinase (MEK) and extracellular signal regulated kinase (ERK) signaling pathways.Methods:Totally 60 SD rats were divided into control group (10 rats) and model group (50 rats). A coronary heart disease model was established in modeling rats. The successfully modeled rats were divided into model group, drug control group, ginseng polysaccharide high- and low-dosage groups, with 10 rats in each group. The drug control group was orally administered with 2 mg/kg Lipitor solution, while the ginseng polysaccharide low- and high-dosage groups were orally administered with 100 and 200 mg/kg ginseng polysaccharide solution. The control group and model group were orally administered with equal volumes of physiological saline solution once a day for one week. HE staining and Masson staining were used to observe the morphological changes and pathological morphology of myocardial tissue. ELISA was used to detect the levels of NO, eNOS, ET-1, ADMA, TNF-α, CRP, and IL-6. Immunochemiluminescence was used to detect the expression levels of CK-MB and cTnl. Real time fluorescence quantification was used to identify the expression of MEK and ERK. Western blot was used to detect the expression of MEK, ERK, p-MEK, and p-ERK in myocardial tissue.Results:Compared with the model group, the levels of serum NO and eNOS increased in the drug control group and ginseng polysaccharide low- and high-dosage groups ( P<0.05), while the levels of ET-1, ADMA, TNF-α, CRP, IL-6, CK-MB, and cTnl decreased ( P<0.05), and the mRNA and protein expressions of MEK and ERK in myocardial tissue decreased ( P<0.05). Conclusions:Ginseng polysaccharides can improve the degree of myocardial fibrosis in rats, alleviate endothelial function and myocardial injury. Its mechanism may be related to the inhibition of the MEK/ERK signaling pathway.
