Exploration on the intervention mechanism of Zhuanggu Zhitong Capsules in postmenopausal osteoporosis based on JNK signaling molecules
10.3760/cma.j.cn115398-20241118-00213
- VernacularTitle:基于JNK信号通路探讨壮骨止痛胶囊对绝经后骨质疏松症的干预机制
- Author:
Meihua WU
1
;
Ronghui LI
;
Yunfeng YU
;
Bing GUO
;
Guomin ZHANG
;
Qinghu HE
;
Xiaoming LEI
;
Xinbin XIA
Author Information
1. 湖南中医药大学研究生院，长沙 410208
- Keywords:
Osteoporosis, postmenopausal;
Zhuanggu Zhitong Capsule;
Alendronate sodium;
JNK signal molecu;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(5):630-637
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Zhuanggu Zhitong Capsules on JNK signaling molecules and their phosphorylated proteins in postmenopausal osteoporosis model female mice.Methods:The rats were divided into sham-operation group, blank group, model group, positive drug group, and Zhuanggu Zhitong Capsules group according to the random number table method, with 10 rats in each group. The model group, the positive drug group and the Zhuanggu Zhitong Capsules group were prepared by bilateral ovarian detomy to prepare a female mouse model of postmenopausal osteoporosis. The positive drug group was given 0.9 mg/kg of alendronate sodium, the Zhuanggu Zhitong Capsules group was given was Zhuanggu Zhitong Capsules 1.944 g/kg for gavage, and the blank group, sham-operation group, and model group were given the same volume of normal saline for gavage, once a day for a total of 13 weeks. Rat vaginal exfoliated cells were stained with Wright's staining; serum Omentin-1 and 25(OH)D 3 levels were determined by ELISA; renal tissue and femoral structure were observed by HE staining; JNK and p-JNK protein expressions were detected by immunohistochemical staining; JNK mRNA levels were detected by PCR. Results:Compared with the model group, the serum levels of 25(OH)D3 and Omentin-1 in the Zhuanggu Zhitong Capsules group and the positive drug group increased ( P<0.01), the mean gray values of JNK and p-JNK protein in bone and kidney tissues decreased ( P<0.01), and the mRNA levels of JNK in bone and kidney tissues decreased ( P<0.01). Conclusion:Zhuanggu Zhitong Capsules can effectively improve the bone microstructure of postmenopausal osteoporotic rats, and its mechanism may be related to the regulation of JNK signaling pathway.
