Establishment of HPLC fingerprint of Gentiana rigescens and determination of four iridoid glycosides
10.3760/cma.j.cn115398-20240717-00183
- VernacularTitle:坚龙胆药材HPLC指纹图谱建立及4种环烯醚萜苷含量测定
- Author:
Zhenyu LI
1
;
Yueyi LIANG
;
Jie YANG
;
Tianrui XIA
;
Fangping ZHANG
;
Roushan CHEN
;
Zhipeng CHEN
;
Lin ZHOU
;
Xiangdong CHEN
;
Dongmei SUN
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Assay (TCD);
Gentiana rigescens;
HPLC fingerprint;
Chemometrics;
Iridoid glycosides
- From:
International Journal of Traditional Chinese Medicine
2025;47(4):522-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish HPLC fingerprint and methods for determining the contents of four iridoid glycosides of Gentiana rigescens; To evaluate the quality of Gentiana rigescens from different origins; To improve the quality control level of Gentiana rigescens medicinal materials.Methods:Using 15 batches of Gentiana rigescens from the main production areas and authentic production areas as raw materials, the common mode of HPLC fingerprints of Gentiana rigescens was established, and the chemical components of the common peaks were identified. Referring to the common mode of fingerprints, similarity analysis was conducted on the fingerprints of Gentiana rigescens from different origins. Using chemometric methods, cluster analysis (HCA), principal component analysis (HCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed on 15 batches of Gentiana rigescens, with the common peak area of fingerprint as the variable. The contents of four types of iridoid glycosides in Gentiana rigescens were determined. Combined with the fingerprints and the content results of four types of iridoid glycosides, the quality of Gentiana rigescens from different origins was evaluated.Results:The fingerprints of Gentiana rigescens contained 9 common peaks, with 4 identified iridoid glycosides. The similarity of the fingerprints of 15 batches of Gentiana rigescens ranged from 0.962 to 0.999. HCA and PCA divided the 15 batches of Gentiana rigescens into two categories. OPLS-DA analyzed 3 significantly different components, namely gentiopicroside, peak 7, and loganic acid. The content determination results showed that the average contents of loganic acid, swertiamarin, and gentiopicroside in Gentiana rigescens from Dali Bai Autonomous Prefecture and Yunnan Province were the highest, and the total amount of four iridoid glycosides was also significantly higher than that from other regions, indicating that the overall quality of Gentiana rigescens from Dali Bai Autonomous Prefecture and Yunnan Province was relatively good.Conclusion:This method is simple, fast, accurate, and can provide reference for improving the quality standards of Gentiana rigescens.
