Study on HPLC fingerprint and determination of two components in Qigong Pills
10.3760/cma.j.cn115398-20240730-00313
- VernacularTitle:启宫丸HPLC指纹图谱建立及2个成分含量测定研究
- Author:
Xiaoshan WANG
1
;
Lei LI
;
Hongyang ZHANG
;
Yongxi WANG
;
Qinpeng WANG
Author Information
1. 赤峰学院附属医院中医科，赤峰 024000
- Keywords:
Assay (TCD);
Qigong Pill;
Fingerprint;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2025;47(4):510-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish fingerprints and method for determining the content of two components of Qigong Pills.Methods:High performance liquid chromatography (HPLC) was used to establish the fingerprints of 10 batches of Qigong Pills and common peaks were identified. TCM Chromatographic Fingerprint Similarity Evaluation System (2012 Edition) was used for similarity analysis; the common peaks area was used as indicators, and SIMCA 14.1 was used for cluster analysis and principal component analysis for quality evaluation; a content analysis method was established using hesperidin and Atractylodes macrocephala as indicator components.Results:21 common peaks in the 10 batches of Qigong Pills were identified with the similarity greater than 0.952 (except for the sample S6); the clustering analysis results showed that at a measurement distance of 8, S6 was clustered into one category alone, S1 and S2 were clustered into one category, and the remaining samples were clustered into one major category. The content of hesperidin and Atractylodes macrocephala in 10 batches of Qigong Pills was 3.61-8.92 mg/g and 0.07-0.72 mg/g, respectively.Conclusion:The established HPLC fingerprints and content determination method in this study are stable, reliable, and reproducible, which can provide reference for quality control and evaluation of Qigong Pills.
