Exploration on effects of Bushen Antai Mixture on placental oxidative damage in mice with RSA investigation based on Nrf2/HO-1/NF-κB signaling pathways
10.3760/cma.j.cn115398-20240728-00304
- VernacularTitle:基于Nrf2/HO-1/NF-κB信号通路探讨补肾安胎合剂对复发性流产小鼠胎盘氧化损伤的影响
- Author:
Haijing CHU
1
;
Chengcheng LIANG
;
Zhe LI
;
Heng HU
;
Jing YU
;
Jijun CHU
Author Information
1. 安徽中医药大学第一临床医学院，合肥 230012
- Keywords:
Abortion, threatened;
Bushen Antai Granules;
Nrf2;
HO-1;
NF-kappa B;
Mice
- From:
International Journal of Traditional Chinese Medicine
2025;47(4):494-500
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Bushen Antai Mixture on nuclear transcription factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1)/nuclear transcription factor-κB (NF-κB) in placental tissue of recurrent spontaneous abortion (RSA) mice.Methods:The CBA/J mice (female) and DBA/2 (male), CBA/J mice (female) and BALB/c (male) were caged at a ratio of 2 : 1 to establish RSA and normal pregnant mice, respectively. The next morning, the vaginal plug was found or the sperm was seen under the microscope as the successful modeling, which was counted as the first day of pregnancy. CBA/J×BALB/c mice were set as normal group, and the RSA model pregnant mice were divided into model group, progesterone group, and Bushen Antai Mixture low-, medium-, and high-dosage groups using a random number table method, with 6 mice in each group. The normal group and the model group were given distilled water by gavage. Bushen Antai Mixture low-, medium-, and high-dosage groups were given 3.9 g/kg, 11.7 g/kg and 23.4 g/kg Bushen Antai Mixture by gavage. The progesterone group was given progesterone capsule aqueous solution 26 mg/kg by gavage for 14 consecutive days. The embryo loss rate of mice was calculated. The pathological changes of placental tissues were observed by HE staining. The expressions of Nrf2, HO-1 and NF-κB protein and mRNA in placental tissues were detected by Western blot and RT-qPCR.Results:Compared with the model group, the embryo loss rate of mice in the Bushen Antai Mixture low-, medium-, and high-dosage groups and the progesterone group significantly decreased ( P<0.05, P<0.01); the pathological morphology of placental tissue improved; the expressions of Nrf2, HO-1 protein and mRNA in placental tissue significantly increased ( P<0.05, P<0.01), and the expression of NF-κB protein and mRNA significantly decreased ( P<0.05, P<0.01). Conclusion:Bushen Antai Mixture may may treat RSA by activating the Nrf2/HO-1 pathway, inhibiting NF - κB expression, and improving oxidative stress and inflammatory response in the placental tissue of RSA pregnant mice.
