Effects of electroacupuncture at "Shenting", "Benshen" and "Baihui" acupoints on mechanical pain threshold and PI3K/TRPV1 pathway in trigeminal ganglion in rats with infraorbital nerve chronic constriction injury
10.3760/cma.j.cn115398-20241107-00090
- VernacularTitle:电针“神庭”“本神”“百会”对三叉神经痛模型大鼠机械痛阈及对三叉神经节JAK/PI3K/TRPV1通路的影响
- Author:
Qiuhui SUN
1
;
Yang GAO
;
Jilong WANG
;
Yingzhe SUN
;
Tianyang YU
;
Yuanzheng SUN
Author Information
1. 黑龙江中医药大学第二临床医学院，哈尔滨 150040
- Keywords:
Trigeminal neuralgia;
Electroacupunctrue;
Infraorbital nerve chronic constriction injury;
TRPV1;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(4):488-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of electroacupuncture at "Shenting", "Benshen" and "Baihui" acupoints on the mechanical pain threshold and JAK/PI3K/TRPV1 pathway in the trigeminal ganglion of rats with trigeminal neuralgia model; To explore the related mechanism.Methods:Totally 36 male SD rats were divided into sham-operation group, model group and electroacupuncture group using random number table method, with 12 rats in each group. Except for the sham-operation group, rats in the model group and electroacupuncture group were modeled by infraorbital nerve chronic constriction injury. In the electroacupuncture group, electroacupuncture was performed at "Shenting", "Benshen" and "Baihui" 14 days after surgery, 20 min every day, once every other day, and every 3 times for 1 course of treatment with an interval of 2 d between each course of treatment. A total of 2 courses of treatment were performed. VonFrey fiber wire was used to measure the mechanical pain threshold of rat whisker pads. HE staining was used to observe the morphology and structure of trigeminal ganglion of rats in each group. Immunohistochemistry and Western blot method were used to observe the protein expressions of JAK, PI3K and TRPV1 in trigeminal ganglion of rats, and the serum level of IL-6 was detected in the serum of rats by ELISA.Results:Compared with the model group, the pain threshold of the electroacupuncture group increased significantly ( P<0.05), and the infiltration of inflammatory cells and demyelination in the trigeminal nerve ganglion decreased, and the positive expressions of JAK, PI3K, and TRPV1 in the trigeminal ganglion decreased ( P<0.05 or P<0.01), the protein expressions of JAK2, PI3K, and TRPV1 decreased ( P<0.05 or P<0.01), and the serum IL-6 level decreased ( P<0.01). Conclusions:Electroacupuncture at "Shenting", "Benshen" and "Baihui" may play an analgesic role by regulating IL-6 levels and inhibiting the activation of JAK/PI3K/TRPV1 signaling pathway.
