Effects of electroacupuncture at acupoints of Shaoyang meridian on peripheral TRPV1 and CGRP in rats with chronic migraine
10.3760/cma.j.cn115398-20240710-00116
- VernacularTitle:电针少阳经穴对慢性偏头痛大鼠外周TRPV1及CGRP的影响
- Author:
Runze TU
1
;
Yixiang ZENG
;
Shengli ZHOU
;
Haojia WEN
;
Yang YANG
;
Zhuozhong HE
;
Weiai LIU
;
Ke HE
;
Lei FU
Author Information
1. 湖南中医药大学第二附属医院针灸推拿康复科，长沙 410005
- Keywords:
Migraine;
Electroacupuncture;
Shaoyang meridians;
Peripheral sensitization;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(4):483-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of electroacupuncture (EA) at acupoints of Shaoyang meridian on mechanical and thermal pain threshold, TRPV1, CGRP mRNA and TRPV1, p-TRPV1, CGRP protein expressions in TG of CM rats; To explore its mechanism.Methods:Totally 24 SD rats were divided into blank group, model group and EA group according to random number table method, with 8 rats in each group. The blank group did not do any treatment; the model group and the EA group were given intraperitoneal injection of nitroglycerin (10 mg/kg) on the 1st, 3rd, 5th, 7th and 9th day, once a day, for 5 times to prepare migraine rat model; after modeling, the model group was given binding and restraint for 30 min, and the EA group was given binding and restraint for 30 min treatment; the changes of basic mechanical and thermal pain thresholds of rats before and after modeling and intervention were observed, the mRNA expression levels of TRPV1 and CGRP in TG rats were detected by qPCR, and the protein expression levels of TRPV1, p-TRPV1 and CGRP in TG rats were detected by Western blot method.Results:After intervention, compared with the model group, the periorbital and plantar mechanical pain thresholds and plantar thermal pain thresholds in EA group increased ( P<0.05), the TRPV1 and CGRP mRNA expressions in trigeminal ganglion decreased ( P<0.01), the protein expressions of TRPV1, p-TRPV1 and CGRP decreased ( P<0.05). Conclusion:EA at acupoints of Shaoyang meridian can obviously increase the mechanical and thermal pain threshold and improve pain sensitization in CM rats. The mechanism would be related to down-regulating the expression levels of TRPV1 and CGRP mRNA and TRPV1, p-TRPV1 and CGRP protein in the TG of CM rats.
