Study on the quantity value transfer of total arsenic and inorganic arsenic in Hirudo, Hirdo processed product, Standard Decoction, Intermediates and Formula Granules
10.3760/cma.j.cn115398-20240709-00096
- VernacularTitle:水蛭及炮制品、标准汤剂、中间体和配方颗粒总砷、无机砷量值传递研究
- Author:
Huaming LIU
1
;
Mingli MA
;
Xiaoyu LIN
;
Hui ZHANG
;
Pei TAN
Author Information
1. 华润三九医药股份有限公司，深圳 518110
- Keywords:
Hirudo (TCD);
Assay (TCD);
Arsenic;
Transfer rates;
Place of production (TCD)
- From:
International Journal of Traditional Chinese Medicine
2025;47(3):372-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the contents and transfer rates of total arsenic and inorganic arsenic in Hirudo, decoction pieces, standard decoction, intermediates, and formula granules; To provide a basis for the safety evaluation of formula granules of Hirudo.Methods:The inductively coupled plasma mass spectrometry (ICP-MS) and high-performance liquid chromatography-Inductively coupled plasma mass spectrometry (HPLC-ICP-MS) were employed for determining the contents of total arsenic and inorganic arsenic in Hirudo and its scald product. Then the contents, transfer rates, and proportions of inorganic arsenic in total arsenic were analyzed to inform the change rules of total arsenic and inorganic arsenic after processing and decocting. Clustering analysis (CA) was applied by analyzing fifteen batches of Hirudo for identifying the distinction among different producing areas.Results:After processing the Hirudo into Hirudo scald product, the content of arsenic was basically unchanged, arsenite and arsenate were presenting a different variation, the content of arsenite and its proportion in total arsenic decreased, while the content of arsenate and its proportion in total arsenic increased. The total arsenic content was exceeding the standard easily, meanwhile the transfer rate of total arsenic was at high level. The transfer rates of arsenite and arsenate were more than 100%, and the proportions of arsenite and arsenate in total arsenic increased since the decoction pieces were decocted into standard decoction, intermediates and formula granules. CA results showed that producing areas have a certain impact on the arsenic content in Hirudo.Conclusion:This study revealed the change rules of total arsenic and inorganic arsenic in Hirudo after processing and decocting into standard decoction,intermediates, and formula granules, which can providing reference for the production and quality standard formulation of formula granules of Hirudo.
