Study on the serum pharmacochemistry of Fengliaoxing Fengshi Dieda Medicinal Liquor based on UHPLC-Q Exactive Focus MS/MS
10.3760/cma.j.cn115398-20240522-00196
- VernacularTitle:基于UHPLC-Q Exactive Focus MS/MS技术的冯了性风湿跌打药酒血清药物化学研究
- Author:
Tong QU
1
;
Ning LI
;
Hui REN
;
Wenjing LU
;
Xiaomin CUI
;
Jing HU
;
Zhiyong CHEN
Author Information
1. 陕西省中医药研究院中药研究所，西安 710003
- Keywords:
Feng Liao Xing Feng Shi Die Da Yao Jiu;
Serum pharmacochemistry;
Prototype components;
Metabolites
- From:
International Journal of Traditional Chinese Medicine
2025;47(3):357-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the blood-transition prototype components and metabolites of Fengliaoxing Fengshi Dieda medicinal liquor.Methods:Ultra-high performance liquid chromatographyquadrupole/electrostatic field orbital trap high resolution mass spectrometry (UHPLC-Q Exactive Focus MS/MS) technique was used to compare the chromatogram differences of Fengliaoxing Fengshi Dieda medicinal liquor extract, blank serum and drug-containing serum. According to the retention time, relative molecular weight and the ratio with primary and secondary ion fragments provided by MS, the prototype components and metabolites of Fengliaoxing Fengshi Dieda medicinal liquor extract were analyzed in serum of rats after oral administration. The detection conditions were as follows: the mobile phase of methanol (A)-0.1% formic acid solution (B) for elution gradient (0-5 min, 5%A; 5-60 min, 5%-95%A; 60-65 min, 95%A), the flow rate of 0.3 ml/min, heated electrospray ionization, detection range of m/z 80-1 200, positive and negative ion scanning modes.Results:A total of 31 transitional components were detected in the serum, of which 9 were prototype components and 22 were metabolites. The 9 prototype components were identified as phenylacetaldehyde, baogongteng C/ erycibellin, p-coumaric acid, 5-Hydroxymethylfurfural, quinic acid, paeonol, 3-Hydroxybenzaldehyde, salicylic acid, and isourecumenol. The 22 metabolites mainly consist of 11 organic acid components, 3 indole components, 2 organic phenolic components, 2 alkaloid components, 1 nucleoside component, 1 amino acid component, 1 lactone component, and 1 sulfonic component. The metabolic pathways were mainly glucuronidation, sulfation and others, which by phase Ⅱ metabolism.Conclusion:Organic phenols and organic acids are the main components that enter the body of Fengliaoxing Fengshi Dieda Medicinal Liquor, while alkaloid compounds and organic acid components may be potential active ingredients for its pharmacological effects.
