Exploration on the mechanism of Lanqin Granules in the treatment of respiratory tract infection based on UPLC-Q-TOF-MS/MS and network pharmacology
10.3760/cma.j.cn115398-20240520-00171
- VernacularTitle:基于UPLC-Q-TOF-MS/MS和网络药理学探讨蓝芩颗粒治疗呼吸道感染的作用机制
- Author:
Zhenzhen BI
1
;
Gang ZHU
;
Xin ZHOU
;
Jianfang WANG
;
Jianbiao YAO
;
Hao LIU
Author Information
1. 浙江康恩贝制药股份有限公司 浙江省中药制药技术重点实验室，杭州 310052
- Keywords:
Respiratory tract infection;
LanQin Granules;
Tandem mass spectrometry;
Network pharmacology
- From:
International Journal of Traditional Chinese Medicine
2025;47(3):348-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the main chemical components of Lanqin Granules based on UPLC-Q-TOF-MS/MS; To explore the potential targets, core components and related pathways of Lanqin Granules in the treatment of respiratory tract infection through network pharmacology.Methods:Using UPLC-Q-TOF-MS/MS secondary fragment cleavage information, combined with literature and database, the chemical components of Lanqin Granules were analyzed; the related action targets of Lanqin Granules were obtained by PharmMapper; The related targets of respiratory tract infection were obtained from GeneCards and OMIM databases, and the common targets were selected by intersection with the relevant action targets of Lanqin Granules. The common targets were imported into string database, and the protein interaction data were downloaded and input into Cytoscape 3.7.2 software to obtain hub gene; Go function and KEGG pathway enrichment analysis were carried out on DAVID platform, and chemical components closely related to hub gene were obtained by using the Network Anlyzer plug-in.Results:53 chemical constituents of Lanqin Granules were obtained, including 11 alkaloids, 20 flavonoids, 5 terpenoids, 7 organic esters, 2 amino acids and 8 other compounds. 28 hub genes and 38 related core components were obtained. The results of GO analysis showed that the treatment of respiratory tract infection with Lanqin Granules was related to neutrophil degranulation, negative regulation of apoptosis, protein hydrolysis and other biological processes; extracellular exosomes, cytoplasm, extracellular components and other cellular components; the same protein binding, RNA polymerase Ⅱtranscription factor activity, ligand activated sequence specific DNA binding, protein serine/ threonine/ tyrosine kinase activity and other molecular processes. KEGG analysis results mainly involved cancer pathway, lipid and atherosclerosis, metabolic pathway and other signaling pathways.Conclusion:Lanqin Granules can treat respiratory tract infection through multi-component, multi-target and multi-channel, and play the role of anti-inflammatory, antibacterial and anti-virus.
