Effects of electroheating needle on pathologic pain, endoplasmic reticulum stress and EGFR/JAK/STAT signaling pathway in rats with knee osteoarthritis
10.3760/cma.j.cn115398-20240703-00035
- VernacularTitle:电热针对膝骨关节炎大鼠病理性疼痛、内质网应激及EGFR/JAK/STAT信号通路的影响
- Author:
Jie ZHANG
1
;
Mingjun XU
;
Yuanzheng YANG
Author Information
1. 十堰市太和医院/湖北医药学院附属医院针灸科，十堰 442000
- Keywords:
Heated electric needle therapy;
Osteoarthritis, knee;
Pathologic pain;
Endoplasmic reticulum stress;
EGFR/JAK/STAT signaling pathway;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(3):342-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of electroheating on pathologic pain, endoplasmic reticulum stress and EGFR/JAK/STAT signaling pathway in rats with knee osteoarthritis.Methods:Totally 40 SPF SD male rats were divided into normal group (10 rats) and modeling group (40 rats) with random number table method. The model of knee osteoarthritis was prepared by injecting sodium iodoacetate into the joint cavity. The successfully modeled rats were divided into model group, sodium hyaluronate group, and electroheating needle group, with 10 rats in each group. After successful modeling, sodium hyaluronate group was injected with 0.1 ml sodium hyaluronate into joint cavity. The electrothermal acupuncture group was treated with electrothermal acupuncture, and the normal group and the model group were given the same volume of normal saline at the same time, once a day, for two weeks. The mechanical pain threshold (PWT) and thermal pain threshold (PWL) in rats were detected. The pathological morphology of cartilage was detected by HE staining. Immunohistochemical staining was used to detect the expressions of glucose regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) in cartilage tissue. Western blot was used to detect the expressions of EGFR, JAK, and STAT proteins, while RT-PCR was used to detect the mRNA levels of GRP78, CHOP, EGFR, JAK, and STAT in cartilage tissue.Results:The normal group had normal cartilage tissue structure, orderly distribution of synovial cells, neat arrangement of chondrocytes, and no inflammatory cell infiltration. The model group had synovial hyperplasia and hypertrophy, irregular arrangement of synovial cells and a large number of hyperplasia, disordered arrangement of chondrocytes and increased number of inflammatory cell infiltration. Compared with the model group, the PWT and PWL in cartilage tissues of sodium hyaluronate group and electrothermal acupuncture group increased ( P<0.05), the expressions of GRP78 and CHOP in cartilage tissues decreased ( P<0.05), the protein expressions of EGFR, JAK1 and STAT1 in cartilage tissue decreased, and the mRNA expressions of GRP78, CHOP, EGFR, JAK1 and STAT1 in cartilage tissue decreased ( P<0.05). Conclusion:Electroheating needle can improve pathological pain in knee osteoarthritis model rats, and its mechanism may be related to inhibiting endoplasmic reticulum stress and regulating the EGFR/JAK/STAT signaling pathway.
