Discussion of causal relationship between intestinal flora and vitiligo based on "co-diseases of skin and gut" in TCM: two-sample mendelian randomization analysis
10.3760/cma.j.cn115398-20240315-00189
- VernacularTitle:基于中医学“皮肠同病”理论探讨肠道菌群与白癜风的因果关系：两样本孟德尔随机化分析
- Author:
Anning HUANG
1
;
Jianren YANG
;
Jinpeng ZHAO
;
Guomei XU
Author Information
1. 北京中医药大学2022级硕士研究生，北京 100029
- Keywords:
Vitiligo;
Intestinal flora;
Co-diseases of skin and gut;
Causal effect;
Mendelian randomization analysis
- From:
International Journal of Traditional Chinese Medicine
2025;47(3):306-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the causal relationship between intestinal flora and vitiligo by two-sample Mendelian randomization (MR) analysis based on "co-diseases of skin and gut" in TCM.Methods:Genome-wide association study (GWAS) data of intestinal flora samples and vitiligo samples were obtained from the databases of MiBioGen and IEU OpenGWAS Project, respectively. Intestinal flora was used as exposure factor, vitiligo as outcome, and single nucleotide polymorphism (SNP) associated with various intestinal floras was used as instrumental variable. After screening qualified instrumental variable in this study, inverse variance weighting (IVW) was used for MR analysis to investigate the potential causal relationship between intestinal flora and vitiligo, MR-Egger regression, weighted median estimator (WME), weighted mode (WM), and simple mode (SM) were used as supplementary methods for IVW. The Cochran's Q test, MR-Egger intercept test, MR-PRESSO and leave one-out analysis were used for sensitivity analysis.Results:Euryarchaeota (IVW method: OR<1, P<0.05) were the protective factors for the occurrence of vitiligo, and Clostridialesvadin-BB60group (IVW method: OR>1, P<0.05) and Subdoligranulum (IVW method: OR>1, P<0.05) were the risk factors for the occurrence of vitiligo. No heterogeneity effect was found by the Cochran's Q test ( P>0.05), no horizontal pleiotropy was found by the MR-Egger intercept test ( P>0.05), no outliers were found in the MR-PRESSO analysis ( P>0.05), and the results of leave-one-out analysis indicated that the causal effects of the 3 identified intestinal floras on vitiligo were not driven by any single SNP. Conclusions:There are causal effects between some intestinal floras and vitiligo, but the specific mechanisms still need to be further studied. The gut microbiota affects the onset and treatment of vitiligo. Using TCM to regulate the gut microbiota may have a good therapeutic effect on treating vitiligo, providing a direction for clinical diagnosis and treatment of vitiligo.
