Exploration on the mechanism of Modified Shaoyao Decoction in repairing intestinal mucosal barrier of ulcerative colitis through PI3K/Akt/NF-κB pathway
10.3760/cma.j.cn115398-20240805-00035
- VernacularTitle:基于PI3K/Akt/NF-κB通路探讨加味芍药汤对溃疡性结肠炎模型大鼠黏膜屏障修复的作用机制
- Author:
Xinyu ZHAN
1
;
Lei DONG
;
Huiyan XU
;
Haimei ZHANG
;
Yongsen JIA
;
Lingyu KONG
;
Xin YAN
Author Information
1. 华北理工大学中医学院，唐山 063210
- Keywords:
Colitis, ulcerative;
PI3K/Akt/NF-κB;
Modified Shaoyao Decoction;
Intestinal mucosal barrier;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(2):196-204
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of modified Shaoyao Decoction with Astragali Radix and Houttuynize Herba in regulating PI3K/Akt/NF-κB pathway against intestinal mucosal barrier injury in ulcerative colitis (UC) rats.Methods:Totally 72 Wistar rats were randomly divided into a blank control group, a model group, a mesalazine group, and modified Shaoyao Decoction high-, medium-, and low-dose groups using a random number table method. After successful modeling, the mesalazine group was given 0.2 g/kg of mesalazine suspension by gavage, while modified Shaoyao Decoction high-, medium-, and low-dose groups were given 44.5, 22.3, and 11.1 g/kg of modified Shaoyao Decoction by gavage, once a day, for a total of 4 weeks. The pathological changes of colon tissue were observed using HE staining; immunofluorescence assay was used to detect the expressions of tight junction protein (ZO-1), cytoskeletal protein (F-actin), and vascular endothelial cadherin (VE cadherin) in colon tissue; Western blot was used to detect the protein expressions of PI3K, Akt, NF-κB, VEGF, and cyclooxygenase (COX-2) in colon tissue; QRT PCR was used to detect the expressions of PI3K and Akt mRNA in colon tissue; ELISA method was used to detect the levels of serum TNF-α and endothelin (ET).Results:Compared with the model group, the DAI scores of the mesalazine group and modified Shaoyao Decoction high-dosage group decreased significantly on the 7th, 14th, 21st and 28th day after modeling ( P<0.05 or P<0.01). In mesalazine group and modified Shaoyao Decoction high-, medium-, and low-dose groups, the pathological injury score of colon tissue decreased ( P<0.01), the immunofluorescence intensity of ZO-1, F-actin and VE-cadherin protein expression in colon tissue increased ( P<0.05 or P<0.01), and the expressions of PI3K, Akt, NF-κB and VEGF protein decreased ( P<0.01). The expression of COX-2 protein and the levels of PI3K mRNA and Akt mRNA decreased in the mesalazine group and modified Shaoyao Decoction high- and medium groups ( P<0.05 or P<0.01). Conclusion:Modified Shaoyao Decoction can antagonize the intestinal barrier injury of UC by inhibiting PI3K/Akt/NF-κB pathway and promote the healing of intestinal mucosal ulcer.
