Study on the effects of meridian massage of Zhuang medicine on deubiquitination modification of BRCC3 in neuropathic pain rats and its analgesic mechanism
10.3760/cma.j.cn115398-20240615-00138
- VernacularTitle:壮医经筋推拿对神经病理性疼痛大鼠去泛素化酶BRCC3去泛素化修饰的影响及镇痛机制研究
- Author:
Siqi WU
1
;
Sheng ZHENG
;
Shengwen LUO
;
Jiaheng GUAN
;
Yufeng HE
;
Yingye LIANG
;
Wei GAN
Author Information
1. 广西中医药大学研究生院，南宁 530000
- Keywords:
Analgesia;
Meridian tuina of Zhuang medicine;
Neuropathic pain;
Deubiquitination;
Rats
- From:
International Journal of Traditional Chinese Medicine
2025;47(2):190-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the deubiquitination modification of the deubiquitinase BRCC3 in rats with neuropathic pain (NPP) treated with meridian tuina of Zhuang medicine and its analgesic molecular mechanism.Methods:Rats were divided into normal group, sham-operation group, model group, sham-tuina group, and meridian tuina of Zhuang medicine group using a random number table method, with 9 rats in each group. Except for the normal and sham operation groups, spinal nerve ligation models were prepared in all other groups. On the first day after surgery, intervention was carried out on the meridian tuina of Zhuang medicine and sham-tuina groups for 14 days, while the other three groups were not intervened; the paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) of each group of rats were measured at 0 days before operation, 1, 7, and 14 days after operation. Western blot was used to detect the expressions of BRCC3 and NLRP3 proteins in spinal cord tissue, and ELISA was used to detect the level of IL-1β in serum.Results:On postoperative 7 and 14 d, compared with the model group, the meridian tuina of Zhuang medicine group showed an increase in PWMT and PWTL, a decrease in NLPR3 and BRCC3 expression in spinal cord tissue, and a decrease in serum IL-1β levels ( P<0.05). Compared with the sham-tuina group, the meridian tuina of Zhuang medicine group showed an increase in PWMT and PWTL, a decrease in NLRP3 protein expression in spinal cord tissue, and a decrease in IL-1β levels in serum ( P<0.05). Conclusion:Meridian tuina of Zhuang medicine can alleviate pain sensitivity in SNL model rats, and its mechanism is related to the inhibition of the expression of deubiquitinase BRCC3 by meridian massage of Zhuang medicine, which increases the ubiquitination level of NLRP3 and hinders its activation, thereby blocking the immune inflammatory response mediated by inflammatory factors.
