Study on UPLC fingerprint establishment and content determination of Hedyotidis chrysotricahae Herba
10.3760/cma.j.cn115398-20240301-00001
- VernacularTitle:金毛耳草药材UPLC指纹图谱建立及含量测定研究
- Author:
Jianhong HU
1
;
Haiqin ZHOU
;
Jienan CAO
;
Haipei SHI
;
Shengjun CHEN
;
Song LI
;
Feng HAI
;
Kaixue ZHANG
Author Information
1. 江阴天江药业有限公司，江阴 214434
- Keywords:
Assay (TCD);
Hedyotidis chrysotricahae Herba;
Fingerprint;
Cluster analysis;
Principal component analysis;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2025;47(2):229-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study and establish the UPLC fingerprint and multi-index content determination methods of Hedyotidis chrysotricahae Herba; To provide a reference for the quality control of Hedyotidis chrysotricahae Herba.Methods:The chromatographic column was ACQUITY UPLC HSS T3 (100 mm×2.1 mm,1.8 μm). The mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution; the detection wavelength was 254 nm; the flow rate was 0.30 ml/min and column temperature was 35 ℃. The method could determine content and fingerprint of rutin, Kaempferol-3-O-rutinoside, Narcissoside, Neochlorogenic aci, Chlorogenic Acid, Cryptochlorogenic acid and have quality analysis to 17 batches of Hedyotidis chrysotricahae Herba based on the variance of fingerprint, similarity evaluation, clustering analysis along with principal component analysis (PCA) at the same time.Results:The common pattern of UPLC specific chromatogram of Hedyotidis chrysotricahae Herba was established. The 11 common peaks were marked out, among which 7 peaks were identified. 17 batches Hedyotidis chrysotricahae Herba could be divided into 4 categories according to different origins. Quality content of six indicators of Hedyotidis chrysotricahae Herba was in slight difference between different origins, among which the content quality of Hedyotidis chrysotricahae Herba from Duyun in Guizhou Province was the highest.Conclusion:The established UPLC fingerprint and content determination method of 6 indicators from the study can be used for the quality control of Hedyotidis chrysotricahae Herba, which can also provide a theoretical basis for the standard improvement of Hedyotidis chrysotricahae Herba.
