Study on chemical composition and evaluation of the quality of Potentilla anserina L. through fingerprint combined with content determination
10.3760/cma.j.cn115398-20240418-00211
- VernacularTitle:藏药蕨麻化学成分研究及指纹图谱结合含量测定综合评价其质量
- Author:
Guofei CHEN
1
;
Shuijuan LIU
;
Jun CHEN
;
Tian XU
;
Zhisen WANG
Author Information
1. 石家庄藏诺药业股份有限公司藏药质量标准研究室，石家庄 050035
- Keywords:
Assay (TCD);
Potentilla anserina L.;
UPLC-Q-Exactive-MS/MS;
Fingerprint
- From:
International Journal of Traditional Chinese Medicine
2025;47(1):82-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the chemical components of the Tibetan medicinal material Potentilla anserina L. based on UPLC-Q-EXACTIVE-MS/MS; To establish its fingerprint and content determination method combined with UPLC; To provide the basis for its quality evaluation. Methods:Intelligent UPLC-Q-Exactive-MS/MS combined with literature and PubChem database was used to identify chemical components of Potentilla anserina L. HPLC method was used to establish fingerprints of 12 batches of Potentilla anserina L. Chemical pattern analysis was performed to identify markers of difference. The content of quality markers was quantitatively analyzed. Results:14 chemical components were identified from Potentilla anserina L, including 10 flavonoids, 3 oils and 1 proanthocyanidins; 15 common peaks and 7 chemical components were identified from the fingerprints of Potentilla anserina L.; chemical patterns identified 8 difference markers; the content determination results showed that the contents of catechin, Kaji-ichigoside F1, rosamultin, tormentic acid , pomolic acid and ursolic acid in different batches were among 0.028-0.080 mg/g, 0.144-0.361 mg/g, 0.323-0.630 mg/g, 0.233-0.645 mg/g, 0.214-0.384 mg/g, 0.115-0.203 mg/g, respectively. Conclusion:This study can provide a reference for the study of chemical constituents and pharmacodynamic substances of Potentilla anserina L., and provide a scientific basis for the objective evaluation of the quality of Potentilla anserina L. and the improvement of its quality control.
