Analysis on active components and mechanism of Qufeng Ningfei Powder in treating asthma based on UHPLC-Q-TOF/MS and network pharmacology analysis
10.3760/cma.j.cn115398-20240330-00392
- VernacularTitle:基于UHPLC-Q-TOF/MS和网络药理学分析祛风宁肺散治疗哮喘的有效成分和机制
- Author:
Yidan SONG
1
;
Qiuyue LI
;
Guowei DONG
;
Heyin XIAO
;
Kai GUO
;
Xin ZHAO
;
Yanxia CHEN
Author Information
1. 北京中医药大学2021级硕士研究生，北京 100029
- Keywords:
Asthma;
Qufeng Ningfei San;
Network pharmacology;
UHPLC-Q-TOF/MS;
Bioinformatics;
IL-17 signaling pathway
- From:
International Journal of Traditional Chinese Medicine
2025;47(1):64-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the key components and mechanism of Qufeng Ningfei Powder in treating asthma through qualitative analysis of its blood components, combined with network pharmacology and molecular docking techniques.Methods:The blood components of Qufeng Ningfei Powder were qualitatively analyzed using UPLC-QE-Orbitrap-MS technology. R language was employed to analyze chip data from the GEO database, obtaining a list of differentially expressed genes. SwissTargetPrediction was utilized to predict the targets of drug components. Asthma-related targets were searched through databases such as OMIM, GeneCards, and TTD. The intersection of drug and disease targets was identified using Venn online analysis tool, constructing a "drug-component-target-disease" network to screen potential core components. A protein-protein interaction network (PPI) of core targets was constructed using STRING platform and Cytoscape software. GO function enrichment and KEGG pathway analysis were conducted using DAVID database to validate potential mechanism. Molecular docking was performed to verify the interaction between key components and core targets.Results:A total of 64 components were identified, from which 53 active components were screened, corresponding to 609 targets. Further searching disease databases revealed 96 target genes related to asthma, with an intersection of 6 genes between drug and asthma differential genes. Core target gene IL6 and its corresponding core compound were determined through network topology analysis. Molecular docking confirmed the binding of the main active components of Qufeng Ningfei Powder with the core target protein IL6.Conclusion:The blood components of Qufeng Ningfei Powder may regulate IL-17 through IL6, counteract airway remodeling, oxidative stress, and airway hyperresponsiveness, and thus treat asthma.
