Changes and clinical significance of pyroptosis and inflammation indicators in children with acute lymphoblastic leukemia after chemotherapy
10.3969/j.issn.1673-4130.2025.22.004
- VernacularTitle:急性淋巴细胞白血病患儿化疗后细胞焦亡指标与炎症指标变化及临床意义
- Author:
Qiuyan WU
1
;
Hairui SHI
;
Xianhe WANG
;
Yan MEI
;
Yueting LONG
;
Zhiping WU
;
Yihua KONG
Author Information
1. 贵州医科大学第二附属医院检验科,贵州凯里 556000
- Keywords:
acute lymphoblastic leukemia;
pyroptosis;
cytokines;
granulocyte;
infection
- From:
International Journal of Laboratory Medicine
2025;46(22):2710-2714,2720
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes and clinical significance of pyroptosis and inflammation in children with acute lymphoblastic leukemia after chemotherapy.Methods A retrospective analysis was con-ducted on the clinical data of 98 children with acute lymphoblastic leukemia who received chemotherapy in the pediatrics and hematology and oncology departments of the hospital from May 2023 to August 2024.Accord-ing to the results of blood culture,the selected children were divided into the Gram-positive bacteria group,the Gram-negative bacteria group,the fungal group and the non-bloodstream infection group,and drug sensitivity tests were conducted.After chemotherapy,the children were divided into the granulocytosis group and the non-granulocytosis group according to the granulocyte level.The relevant indicators were detected and com-pared by methods such as blood routine,flow microsphere array technology,enzyme-linked immunosorbent assay(ELISA),and Western blot.Results After chemotherapy,the pyroptosis related indicators caspase-1,caspase-4,caspase-5,caspase-11,interleukin(IL)-1 β,IL-18,the proportion of pyroptosis cells and the relative expression level of GSDMD protein in children of each infection type were significantly increased compared with those before chemotherapy(P＜0.05).After chemotherapy,the levels of IL-4,IL-6,IL-10 and interfer-on-γ(IFN-γ)in the granulocytosis group were significantly higher than those in the non-granulocytosis group(P＜0.05),and the granulocyte level was negatively correlated with the levels of IL-4,IL-6,IL-10 and IFN-γ(P＜0.05).There were statistically significant differences in the levels of IL-4,IL-6,IL-10 and IFN-γ in dif-ferent infection states after chemotherapy(P＜0.05).Conclusion The number of granulocytes and the levels of serum cytokines can serve as potential indicators of infection in children with leukemia.The regulation of pyroptosis may provide new strategies for the treatment of childhood leukemia.
