Study on the inhibition of cell cycle progression and proliferation ability of lung adenocarcinoma cells by regulating the TGF-β1/Smads signaling pathway by morin
10.3969/j.issn.1673-4130.2025.20.015
- VernacularTitle:桑色素通过调节TGF-β1/Smads信号通路抑制肺腺癌细胞的细胞周期进程及增殖能力的研究
- Author:
Juzheng WANG
1
;
Jiayu LU
;
Qingshi WANG
;
Wen LIU
;
Bo BAO
;
Yiming LI
;
Qiang LU
Author Information
1. 咸阳市第一人民医院胸外科,陕西咸阳 712000
- Keywords:
morin;
TGF-β1/Smads signaling pathway;
cell cycle;
lung cancer
- From:
International Journal of Laboratory Medicine
2025;46(20):2513-2517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of morin on the cell cycle progression and prolif-eration ability of lung adenocarcinoma cells by regulating the transforming growth factor β1(TGF-β1)/Smads signaling pathway.Methods Human lung adenocarcinoma cell line A549 was used for in vitro experiments.Different concentrations of morin(0,30,90,150 μg/mL)were used to treat human lung adenocarcinoma cell line A549.Cell survival rate was detected by CCK-8 method,cell cycle distribution was detected by Hoechst 33342 staining method,cell proliferation ability was detected by clone formation assay,cell invasion ability was detected by Transwell invasion assay,cell apoptosis was detected by TUNEL staining method,and the expres-sion levels of TGF-β1/Smads signaling pathway related proteins were analyzed by Western blot.Results CCK-8 experimental results showed that the survival rate of A549 cells in the morin-treated group significantly decreased with the increase in morin concentration(P＜0.05).The results of Hoechst 33342 staining showed that the proportion of G1 phase cells in the morin-treated group significantly increased,and the proportion of S phase and G2/M phase cells significantly decreased(P＜0.05).The results of the colony formation assay showed that the number of colonies formed in the morin-treated group was significantly less than that in the control group,and the colony formation rates in the 90 μg/mL and 150 μg/mL groups were reduced by approximately 50％and 70％respectively(P＜0.05).Transwell invasion assay results showed that the number of invasive cells in the morin-treated group was significantly reduced(P＜0.05).TUNEL staining results showed that the proportion of apoptotic cells in the morin-treated group significantly increased(P＜0.05).Western blot results showed that the expression levels of TGF-β1 and p-Smad2/3 in the morin-treated group were significantly reduced(P＜0.05),and the expression level of Smad7 was significantly in-creased(P＜0.05).Conclusion Morin blocks A549 cells in the G1 phase by regulating the TGF-β1/Smads signaling pathway,thereby inhibiting cell proliferation,reducing cell invasion ability,and inducing cell apopto-sis.This indicates that morin has potential application value in the treatment of lung adenocarcinoma.
