Research progress in pathogen nucleic acid detection technology based on the CRISPR/Cas system
10.3969/j.issn.1673-4130.2025.19.017
- VernacularTitle:基于CRISPR/Cas系统的病原体核酸检测技术研究进展
- Author:
Ainiwaer AILIFEIRE
1
;
Yating NING
;
Yingchun XU
;
Li ZHANG
Author Information
1. 中国医学科学院北京协和医学院/北京协和医院疑难重症及罕见病国家重点实验室检验科,北京 100730;中国医学科学院北京协和医学院研究生院,北京 100005
- Keywords:
clustered regularly interspaced short palindromic repeats/CRISPR-associated protein system;
Cas protein;
nucleic acid testing;
pathogenic microorganisms;
diagnosis
- From:
International Journal of Laboratory Medicine
2025;46(19):2408-2414,2420
- CountryChina
- Language:Chinese
-
Abstract:
Infectious diseases caused by bacteria,viruses,and other pathogens are a significant global public health challenge.Accurate and efficient detection methods are crucial for the diagnosis and prevention of infec-tious diseases.Nucleic acid detection technologies based on the clustered regularly interspaced short palin-dromic repeats(CRISPR)and CRISPR-associated protein(Cas)systems have begun to be applied in the de-tection of pathogenic microorganisms due to their high sensitivity,high specificity,and rapid simplicity.In re-cent years,various convenient and efficient nucleic acid detection technologies and platforms for pathogenic microorganisms have been derived from the CRISPR/Cas system,further expanding the field of pathogen nu-cleic acid detection.This article summarizes the research progress of pathogen nucleic acid detection technolo-gies based on the CRISPR/Cas system,discusses the value of these detection technologies in the in vitro diag-nosis of pathogens,and provides references for the further development of CRISPR/Cas nucleic acid detection technologies.
